In Vivo Antiplasmodial Activity of Terminalia mantaly Stem Bark Aqueous Extract in Mice Infected by Plasmodium berghei
| Autor: | Issakou Bakarnga-Via, Alvine Ngoutane Mfopa, Raceline Gounoue Kamkumo, Cedric Derick Jiatsa Mbouna, Fabrice Fekam Boyom, Mariscal Brice Tchatat Tali, Jaures Marius Tsakem Nangap, Rodrigue Keumoe, Lauve R. Y. Tchokouaha, Patrick Valere Tsouh Fokou |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Traditional medicine
Article Subject 030231 tropical medicine Infectious and parasitic diseases RC109-216 Biology biology.organism_classification 01 natural sciences Median lethal dose In vitro Acute toxicity 0104 chemical sciences 010404 medicinal & biomolecular chemistry 03 medical and health sciences 0302 clinical medicine Infectious Diseases In vivo Maceration (wine) Parasitology Plasmodium berghei IC50 ED50 Research Article |
| Zdroj: | Journal of Parasitology Research Journal of Parasitology Research, Vol 2020 (2020) |
| ISSN: | 2090-0031 2090-0023 |
| Popis: | Background.Terminalia mantalyis used in Cameroon traditional medicine to treat malaria and related symptoms. However, its antiplasmodial efficacy is still to be established.Objectives. The present study is aimed at evaluating thein vitroandin vivoantiplasmodial activity and the oral acute toxicity of theTerminalia mantalyextracts.Materials and Methods. Extracts were prepared from leaves and stem bark ofT. mantaly, by maceration in distilled water, methanol, ethanol, dichloromethane (DCM), and hexane. All extracts were initially screenedin vitroagainst the chloroquine-resistant strain W2 ofP. falciparumto confirm itsin vitroactivity, and the most potent one was assessed in malaria mouse model at three concentrations (100, 200, and 400 mg/kg/bw). Biochemical, hematological, and histological parameters were also determined.Results. Overall, 7 extracts showedin vitroantiplasmodial activity with IC50ranging from 0.809 μg/mL to 5.886 μg/mL. The aqueous extract from the stem bark ofT. mantaly(Tmsbw) was the most potent (IC50=0.809 μg/mL) and was further assessed for acute toxicity and efficacy inPlasmodium berghei-infected mice.Tmsbwwas safe in mice with a median lethal dose (LD50) higher than 2000 mg/kg of body weight. It also exerted a good antimalarial efficacyin vivowith ED50of 69.50 mg/kg and had no significant effect on biochemical, hematological, and histological parameters.Conclusion. The results suggest that the stem bark extract ofT. mantalypossesses antimalarial activity. |
| Databáze: | OpenAIRE |
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