Role of Ser289 in RARγ and Its Homologous Amino Acid Residue of RARα and RARβ in the Binding of Retinoic Acid
| Autor: | Jerome L. Gabriel, Zeng-Ping Zhang, Kenneth J. Soprano, Dianne Robert Soprano, Muna Shukri, Carlo J. Gambone |
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| Rok vydání: | 2000 |
| Předmět: |
Transcriptional Activation
Receptors Retinoic Acid medicine.drug_class Biophysics Retinoic acid Tretinoin In Vitro Techniques Biochemistry Mice chemistry.chemical_compound Transactivation Serine Homologous chromosome medicine Animals Retinoid Amino acid residue Receptor Molecular Biology DNA Primers Binding Sites Base Sequence Retinoic Acid Receptor alpha Ligand binding domain Recombinant Proteins Protein Structure Tertiary Cell biology Kinetics chemistry Mutagenesis Site-Directed |
| Zdroj: | Archives of Biochemistry and Biophysics. 380:339-346 |
| ISSN: | 0003-9861 |
| DOI: | 10.1006/abbi.2000.1932 |
| Popis: | The biological actions of retinoic acid (RA) are mediated by retinoic acid receptors (RARalpha, -beta, and -gamma) and retinoid X receptors (RXRalpha, -beta, and -gamma). Although the ligand-binding domains of RARs and RXRs have been suggested to share the same novel folding pattern, the ligand-binding pockets of each of the retinoid receptors must have unique structural features since it has been possible to develop RAR subtype-selective and RXR-selective retinoids. We have previously demonstrated the importance for RA binding and RA-dependent transactivation of Arg(276) in RARalpha and Arg(278) in RARgamma; however, in RARbeta Arg(269) functions in conjunction with Lys(220). Here we have examined the role of the hydroxyl group of RARgamma Ser(289) and its homologous amino acid residues in RARalpha (Ser(287)) and RARbeta (Ser(280)) alone and in conjunction with their respective RARgamma Arg(278) homologs for RA binding and RA-dependent transactivation activity. The hydroxyl group of this Ser in all three RARs was found by itself not to be important for RA binding and RA-dependent transactivation activity. However, in RARalpha and RARgamma this Ser appears to play a small role in conjunction with Arg(276) and Arg(278), respectively, for these activities. Alternatively, strong synergism was observed in RARbeta between Ser(280) and Arg(269) for RA-binding and RA-dependent transactivation activity. This provides further evidence that the mechanism of interaction between the carboxylate group of retinoids and the amino acid residues in the ligand binding pocket of RARbeta is different from that of RARalpha and RARgamma. |
| Databáze: | OpenAIRE |
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