Transcriptional profiling of MEF2-regulated genes in human neural progenitor cells derived from embryonic stem cells
| Autor: | Nobuki Nakanishi, Stuart A. Lipton, Rameez Zaidi, Shu-ichi Okamoto, Shing Fai Chan, Xiayu Huang, Scott R. McKercher |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Mef2
Microarray lcsh:QH426-470 Microarray analysis techniques Human neural stem cells Biology Stem cell marker Biochemistry Embryonic stem cell Molecular biology Neural stem cell Cell biology lcsh:Genetics Neuronal differentiation Data in Brief Genetics Molecular Medicine Gene expression Progenitor cell Stem cell MEF2 Transcription factor Biotechnology |
| Zdroj: | Genomics Data, Vol 3, Iss C, Pp 24-27 (2015) Genomics Data |
| ISSN: | 2213-5960 |
| Popis: | [Briefly describe the contents of the Data in Brief article. Tell the reader the repository and reference number for the data in the abstract to.] The myocyte enhancer factor 2 (MEF2) family of transcription factors is highly expressed in the brain, and constitutes a key determinant of neuronal survival, differentiation, and synaptic plasticity. However, genome-wide transcriptional profiling of MEF2-regulated genes has not yet been fully elucidated, particularly at the neural stem cell stage. Here we report the results of microarray analysis comparing mRNAs isolated from human neural progenitor/stem cells (hNPCs) derived from embryonic stem cells expressing a control vector versus progenitors expressing a constitutively-active form of MEF2 (MEF2CA), which increases MEF2 activity. Microarray experiments were performed using the Illumina Human HT-12 V4.0 expression beadchip (GEO#: GSE57184). By comparing vector-control cells to MEF2CA cells, microarray analysis identified 1880 unique genes that were differentially expressed. Among these genes, 1121 genes were upregulated and 759 genes were down-regulated. Our results provide a valuable resource for identifying transcriptional targets of MEF2 in hNPCs. |
| Databáze: | OpenAIRE |
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