Combined use ofl-alanine tert butyl ester lactate and trimethyl-β-cyclodextrin for the enantiomeric separations of 2-arylpropionic acids nonsteroidal anti-inflammatory drugs

Autor: Mavroudi, Maria C., Kapnissi‐Christodoulou, Constantina P.
Přispěvatelé: Kapnissi‐Christodoulou, Constantina P. [0000-0003-3755-1052]
Rok vydání: 2015
Předmět:
Ketoprofen
analytical parameters
lactic acid derivative
Clinical Biochemistry
Flurbiprofen
chirality
Biochemistry
Analytical Chemistry
chemistry.chemical_compound
nonsteroid antiinflammatory agent
beta cyclodextrin derivative
ibuprofen
chemistry.chemical_classification
Fenoprofen
Alanine
Phenylpropionates
Cyclodextrin
Anti-Inflammatory Agents
Non-Steroidal

beta-Cyclodextrins
Nonsteroidal anti-inflammatory drugs
fenoprofen
Stereoisomerism
CD
unclassified drug
acetic acid
alanine tert butyl ester lactate
Lactates
arylpropionic acid derivative
alanine
stereoisomerism
medicine.drug
Naproxen
ketoprofen
phenylpropionic acid derivative
trimethyl beta cyclodextrin
capillary electrophoresis
chemistry
Article
medicine
naproxen
procedures
reproducibility
indoprofen
Dual separation system
separation technique
Chromatography
concentration (parameters)
isolation and purification
Reproducibility of Results
temperature
Electrophoresis
Capillary

Indoprofen
Chiral separation
flurbiprofen
carprofen
Amino acid ester-based ionic liquid
Enantiomer
Sodium acetate
Zdroj: Electrophoresis
ISSN: 0173-0835
DOI: 10.1002/elps.201500143
Popis: In this study, a new CE method, employing a binary system of trimethyl-ß-CD (TM-ß-CD) and a chiral amino acid ester-based ionic liquid (AAIL), was developed for the chiral separation of seven 2-arylpropionic acid nonsteroidal anti-inflammatory drugs (NSAIDs). In particular, the enantioseparation of ibuprofen, ketoprofen, carprofen, indoprofen, flurbiprofen, naproxen, and fenoprofen was improved significantly by supporting the BGE with the chiral AAIL l-alanine tert butyl ester lactate (l-AlaC4Lac). Parameters, such as concentrations of TM-ß-CD and l-AlaC4Lac, and buffer pH, were systematically examined in order to optimize the chiral separation of each NSAID. It was observed that the addition of the AAIL into the BGE improved both resolution and efficiency significantly. After optimization of separation conditions, baseline separation (Rs>1.5) of five of the analytes was achieved in less than 11 min, while the resolution of ibuprofen and flurbiprofen was approximately 1.2. The optimized enantioseparation conditions for all analytes involve a BGE of 5 mM sodium acetate/acetic acid (pH 5.0), an applied voltage of 30 kV, and a temperature of 20°C. In addition, the results obtained by computing the %-RSD values of the EOF and the two enantiomer peaks, demonstrated excellent run-to-run, batch-to-batch, and day-to-day reproducibilities. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. 36 19 2442 2450 Manufacturers: Fluka, Germany Cited By :7
Databáze: OpenAIRE