Combined use ofl -alanine tert butyl ester lactate and trimethyl-β-cyclodextrin for the enantiomeric separations of 2-arylpropionic acids nonsteroidal anti-inflammatory drugs
| Autor: | Mavroudi, Maria C., Kapnissi‐Christodoulou, Constantina P. |
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| Přispěvatelé: | Kapnissi‐Christodoulou, Constantina P. [0000-0003-3755-1052] |
| Rok vydání: | 2015 |
| Předmět: |
Ketoprofen
analytical parameters lactic acid derivative Clinical Biochemistry Flurbiprofen chirality Biochemistry Analytical Chemistry chemistry.chemical_compound nonsteroid antiinflammatory agent beta cyclodextrin derivative ibuprofen chemistry.chemical_classification Fenoprofen Alanine Phenylpropionates Cyclodextrin Anti-Inflammatory Agents Non-Steroidal beta-Cyclodextrins Nonsteroidal anti-inflammatory drugs fenoprofen Stereoisomerism CD unclassified drug acetic acid alanine tert butyl ester lactate Lactates arylpropionic acid derivative alanine stereoisomerism medicine.drug Naproxen ketoprofen phenylpropionic acid derivative trimethyl beta cyclodextrin capillary electrophoresis chemistry Article medicine naproxen procedures reproducibility indoprofen Dual separation system separation technique Chromatography concentration (parameters) isolation and purification Reproducibility of Results temperature Electrophoresis Capillary Indoprofen Chiral separation flurbiprofen carprofen Amino acid ester-based ionic liquid Enantiomer Sodium acetate |
| Zdroj: | Electrophoresis |
| ISSN: | 0173-0835 |
| DOI: | 10.1002/elps.201500143 |
| Popis: | In this study, a new CE method, employing a binary system of trimethyl-ß-CD (TM-ß-CD) and a chiral amino acid ester-based ionic liquid (AAIL), was developed for the chiral separation of seven 2-arylpropionic acid nonsteroidal anti-inflammatory drugs (NSAIDs). In particular, the enantioseparation of ibuprofen, ketoprofen, carprofen, indoprofen, flurbiprofen, naproxen, and fenoprofen was improved significantly by supporting the BGE with the chiral AAIL l-alanine tert butyl ester lactate (l-AlaC4Lac). Parameters, such as concentrations of TM-ß-CD and l-AlaC4Lac, and buffer pH, were systematically examined in order to optimize the chiral separation of each NSAID. It was observed that the addition of the AAIL into the BGE improved both resolution and efficiency significantly. After optimization of separation conditions, baseline separation (Rs>1.5) of five of the analytes was achieved in less than 11 min, while the resolution of ibuprofen and flurbiprofen was approximately 1.2. The optimized enantioseparation conditions for all analytes involve a BGE of 5 mM sodium acetate/acetic acid (pH 5.0), an applied voltage of 30 kV, and a temperature of 20°C. In addition, the results obtained by computing the %-RSD values of the EOF and the two enantiomer peaks, demonstrated excellent run-to-run, batch-to-batch, and day-to-day reproducibilities. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. 36 19 2442 2450 Manufacturers: Fluka, Germany Cited By :7 |
| Databáze: | OpenAIRE |
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