Non-invasive fecal metabonomic detection of colorectal cancer
| Autor: | Peh Yean Cheah, Han Kiat Ho, Xiu Ping Chue, Lee Cheng Phua, Eric Chun Yong Chan, Poh Koon Koh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Oncology
Male Cancer Research medicine.medical_specialty Colorectal cancer Biology Bioinformatics medicine.disease_cause Feces Metabolomics Internal medicine Metabolome medicine Biomarkers Tumor Humans Aged Pharmacology Aged 80 and over OPLS Receiver operating characteristic Case-control study Middle Aged medicine.disease Case-Control Studies Molecular Medicine Female Carcinogenesis Colorectal Neoplasms Research Paper |
| Popis: | Colorectal cancer (CRC) is a major cause of mortality in many developed countries. Effective screening strategies were called for to facilitate timely detection and to promote a better clinical outcome. In this study, the role of fecal metabonomics in the non-invasive detection of CRC was investigated. Gas chromatography/time-of-flight mass spectrometry (GC/TOFMS) was utilized for the metabolic profiling of feces obtained from 11 CRC patients and 10 healthy subjects. Concurrently, matched tumor and normal mucosae surgically excised from CRC patients were profiled. CRC patients were differentiated clearly from healthy subjects based on their fecal metabonomic profiles (orthogonal partial least squares discriminant analysis [OPLS-DA], 1 predictive and 3 Y-orthogonal components, R (2)X = 0.373, R (2)Y = 0.995, Q (2) [cumulative] = 0.215). The robustness of the OPLS-DA model was demonstrated by an area of 1 under the receiver operator characteristic curve. OPLS-DA revealed fecal marker metabolites (e.g., fructose, linoleic acid, and nicotinic acid) that provided novel insights into the tumorigenesis of CRC. Interestingly, a disparate set of CRC-related metabolic aberrations occurred at the tissue level, implying the contribution of processes beyond the direct shedding of tumor cells to the fecal metabotype. In summary, this work established proof-of-principle for GC/TOFMS-based fecal metabonomic detection of CRC and offered new perspectives on the underlying mechanisms. |
| Databáze: | OpenAIRE |
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