High pathogenicity and low genetic evolution of avian paramyxovirus type I (Newcastle disease virus) isolated from live bird markets in Uganda
| Autor: | Mariette F. Ducatez, Halid Kirunda, Maxwell O Otim, Jessica L. Nakavuma, Kizito K. Mugimba, Angélique Teillaud, Denis K. Byarugaba, Mathilde Paul, John Bosco Omony, Agnes Wanyana, Martin Okitwi |
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| Přispěvatelé: | Makerere University [Kampala, Ouganda] (MAK), Uganda National Council of Science and Technology, Partenaires INRAE, National Livestock Resources Research Institute, Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Veterinary medicine
040301 veterinary sciences [SDV]Life Sciences [q-bio] Newcastle Disease Molecular Sequence Data Newcastle disease virus Virulence Disease Biology Newcastle disease Virus Genetic diversity Poultry maladie de newcastle 0403 veterinary science Evolution Molecular 03 medical and health sciences Virology pseudopeste aviaire Genotype Genetic variation oiseau Pathogenicity Animals Uganda pathogénèse Phylogeny 030304 developmental biology 2. Zero hunger 0303 health sciences Live bird markets Research Commerce Outbreak Genetic Variation 04 agricultural and veterinary sciences biology.organism_classification Infectious Diseases newcastle disease virus live bird markets genetic diversity pathogenicity uganda production de volaille diversité génétique |
| Zdroj: | Virology Journal Virology Journal, BioMed Central, 2014, 11, 13 p. ⟨10.1186/1743-422X-11-173⟩ Virology Journal (11), 13 p.. (2014) |
| ISSN: | 1743-422X |
| Popis: | International audience; Background: Newcastle disease is still a serious disease of poultry especially in backyard free-range production systems despite the availability of cross protective vaccines. Healthy-looking poultry from live bird markets have been suspected as a major source of disease spread although limited studies have been conducted to ascertain the presence of the virulent strains in the markets and to understand how they are related to outbreak strains. Methods: This study evaluated the occurrence of Newcastle disease virus in samples collected from poultry in live bird markets across Uganda. The isolates were pathoyped using standard methods (mean death time (MDT), intracelebral pathogenicity index (ICPI), and sequencing of the fusion protein cleavage site motif) and also phylogenetically analysed after sequencing of the full fusion and hemagglutin-neuraminidase genes. The isolates were classified into genotypes and subgenotypes based on the full fusion protein gene classification system and compared with other strains in the region and world-wide. Results: Virulent avian paramyxovirus type I (APMV-1) (Newcastle disease virus) was isolated in healthy-looking poultry in live bird markets. The viruses belonged to a new subgenotype, Vd, in genotype V, and clustered together with Tanzania and Kenya strains. They harbored low genetic diversity. Conclusion: The occurrence of virulent AMPV-1 strains in live bird markets may serve as sources of Newcastle disease outbreaks in non-commercial farms. |
| Databáze: | OpenAIRE |
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