Characterization of the Inner Enamel Epithelium in the Enamel-Free Area Based on the Ability to Secrete Enamel Protein Demonstrated by in situ Hybridization and Immunohistochemistry
| Autor: | E.F. Sato, Hitoyata Shimokawa, K. Ishizeki, Tokio Nawa, Hitoshi Yamamoto, Junzo Sasaki, T. Nomura, Junko Matsuura |
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| Rok vydání: | 1997 |
| Předmět: |
Histology
Molecular Sequence Data In situ hybridization Biology Matrix (biology) Epithelium Immunoenzyme Techniques Rats Sprague-Dawley Extracellular matrix Dental Enamel Proteins stomatognathic system Pregnancy Dentin medicine Animals RNA Messenger In Situ Hybridization Amelogenin Base Sequence Enamel paint Inner enamel epithelium Enamel Organ food and beverages Molar Molecular biology Extracellular Matrix Rats medicine.anatomical_structure Animals Newborn visual_art visual_art.visual_art_medium Female Anatomy Ameloblast |
| Zdroj: | Cells Tissues Organs. 160:232-238 |
| ISSN: | 1422-6421 1422-6405 |
| DOI: | 10.1159/000148016 |
| Popis: | Both the expression of amelogenin mRNA and secretion of amelogenin were investigated in rat molars by in situ hybridization and immunohistochemistry. Probes were designed by multiple-labeling of oligonucleotide probes for in situ hybridization. Amelogenin mRNA first appeared in differentiating ameloblasts of the distal region and some inner enamel epithelial cells of enamel-free area (EFA cells) of the second cusp at postnatal day 0. At the same time, amelogenin protein was detected in the extracellular matrix between dentin and differentiating ameloblasts and in some EFA cells of the second cusp. At postnatal day 1–3, amelogenin was expressed in the secretory ameloblasts, and in the matrix beneath these cells. Both amelogenin mRNA and amelogenin were detected in the EFA cells and their extracellular matrix. After postnatal day 5, amelogenin mRNA and amelogenin were detected in the secretory ameoloblasts and extracellular matrix in the enamel-forming region, respectively. At this time, amelogenin mRNA was not detected in the EFA cells, but a small amount of amelogenin was found in the matrix beneath the EFA cells. These findings suggest that EFA cells differentiate into amelogenin-secreting cells, i.e. ameloblasts, but that the secretion lasts for only a short period at the early stage of tooth development. |
| Databáze: | OpenAIRE |
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