Comparison of percentage of CD90-positive cells and osteogenic differentiation potential between mesenchymal stem cells grown on dish and nonwoven fabric
| Autor: | Bo Fu, Masashi Fujiwara, Mutsumi Takagi |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Scaffold Nonwoven fabric Chemistry Clinical Biochemistry Mesenchymal stem cell Biomedical Engineering Bioengineering Cell Biology Cell biology Extracellular matrix 03 medical and health sciences 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis Original Article CD90 Biotechnology |
| Zdroj: | Cytotechnology |
| ISSN: | 1573-0778 0920-9069 |
| DOI: | 10.1007/s10616-020-00390-y |
| Popis: | Although nonwoven fabric (NWF) has been reported to be a candidate scaffold for the large-scale expansion of mesenchymal stem cells (MSCs), the quality of cells grown in NWF has not been well clarified. In this report, MSCs grown in an NWF disc for 3 weeks showed higher osteogenic differentiation potential and percentage of CD90 (+) cells than MSCs grown on the bottom surface of dish. The amount of the extracellular matrix (ECM) per unit surface area of fibers was larger than that on the bottom surface of the dish in the first 2 weeks of culture. The osteogenic differentiation potential of MSCs inoculated onto cell-free ECM increased with increasing amount of ECM. The higher percentage of CD90 (+) cells and osteogenic differentiation potential of cells grown in an NWF disc than of cells grown on a dish might, at least in part, be due to the higher amount of ECM. |
| Databáze: | OpenAIRE |
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