Isolation of secretory vesicles from Saccharomyces cerevisiae
| Autor: | Tina Etcheverry, Randy Schekman, Cherie L. Holcomb |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Acid Phosphatase
Saccharomyces cerevisiae Biophysics Spheroplasts Sulfur Radioisotopes Biochemistry Fungal Proteins Organelle Centrifugation Density Gradient Centrifugation Molecular Biology Gel electrophoresis biology Vesicle Sodium Dodecyl Sulfate Cell Biology biology.organism_classification Secretory Vesicle Yeast Microscopy Electron Membrane Mutation Electrophoresis Polyacrylamide Gel Spectrophotometry Ultraviolet |
| Zdroj: | Analytical Biochemistry. 166:328-334 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(87)90581-1 |
| Popis: | Purification of secretory vesicles from Saccharomyces cerevisiae has been hindered because these organelles normally represent a small proportion of cellular membranes. In the yeast secretory mutant sec1, secretory vesicles accumulate intracellularly in large quantities. Using a sec1 strain we have devised a procedure for the partial purification of these vesicles. The purification employs differential and density gradient centrifugations and an electrophoretic separation of membranes. The fractions obtained from this procedure are enriched for secretory vesicles at least fivefold over other cellular membranes. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of solubilized membrane fractions reveals a distinct set of polypeptides associated with secretory vesicles. |
| Databáze: | OpenAIRE |
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