Interaction of influenza A virus NS2/NEP protein with the amino-terminal part of Nup214
| Autor: | Kadir Turan, Atsushi Kawaguchi, Burçak Şenbaş Akyazi, Kyosuke Nagata, Ayşegül Pirinçal |
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| Přispěvatelé: | Senbas Akyazi, Burcak, Pirincal, Aysegul, Kawaguchi, Atsushi, Nagata, Kyosuke, Turan, Kadir |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Nup214
Physiology viruses 0206 medical engineering NUCLEAR-PORE COMPLEX 02 engineering and technology Biology medicine.disease_cause Microbiology Article 03 medical and health sciences Influenza A viruses NS2/NEP Nup214 nuclear pore complex nuclear export 0302 clinical medicine DOMAIN nuclear pore complex Genetics Influenza A virus medicine Nuclear pore Nuclear export signal Molecular Biology Nucleoporin 214 M1 PROTEIN Influenza A viruses FUNCTIONAL-ANALYSIS IMPORT NUCLEOCYTOPLASMIC TRANSPORT POLYMERASE virus diseases RNA Cell Biology biochemical phenomena metabolism and nutrition 020601 biomedical engineering Cell biology Nucleoprotein Viral replication 030220 oncology & carcinogenesis Nucleoporin nuclear export OVEREXPRESSION NUCLEOPORIN NUP214 EXPORT PROTEIN General Agricultural and Biological Sciences NS2/NEP |
| Zdroj: | Volume: 44, Issue: 2 82-92 Turkish Journal of Biology |
| ISSN: | 1300-0152 1303-6092 |
| Popis: | Influenza A viruses have a single-stranded RNA genome consisting of 8 segments. Each RNA segment associates with the nucleoprotein NP and viral RNA polymerase to and from a viral ribonucleoprotein vRNP particle. The viral mRNA synthesis is dependent on a capped primer derived from nascent host RNA transcripts. For these processes to take place, vRNPs must pass through the cell nuclear pore complex NPC to the nucleus. The influenza A virus NS2 protein, also called the nuclear export protein NES , has an important role in the nucleocytoplasmic transport of vRNPs. This protein interacts with the host cellular nucleoporins during the nuclear export of vRNPs. In this study, the human nucleoporin 214 Nup214 was identified as an NS2-binding protein by using a yeast two-hybrid assay. The interaction between NS2 and human Nup214 was confirmed in both yeast and mammalian cells. It has been shown that the NS2 protein interacts with the amino terminal FG domain of the Nup214 protein. The influenza viral replication was suppressed in knockdown cells for the Nup214 protein. It was concluded that the FG domains of nucleoporins have an important role in the interaction of the influenza NS2 protein with host NPC for vRNA export. |
| Databáze: | OpenAIRE |
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