The skin microbiome of caspase-14-deficient mice shows mild dysbiosis
| Autor: | Ken Vercammen, Jens-Michael Schröder, Brigitta M. Brinkman, Jeroen Raes, Jurgen Del Favero, Peter Vandenabeele, Falk Hildebrand, Wim Declercq, Malgorzata Kubica, Dirk Goossens, Pierre Cornelis |
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| Přispěvatelé: | Microbiology, Department of Bio-engineering Sciences |
| Rok vydání: | 2014 |
| Předmět: |
Dermatology
Staphylococcaceae Biochemistry Microbiology Mice chemistry.chemical_compound medicine Animals Caspase 14 Microbiome Molecular Biology Skin Mice Knockout integumentary system biology Catabolism Microbiota Urocanic Acid Atopic dermatitis biology.organism_classification medicine.disease mice caspase-14 moise Disease Models Animal Urocanic acid chemistry Dysbiosis Female Human medicine Filaggrin |
| Zdroj: | Experimental dermatology |
| ISSN: | 0906-6705 |
| DOI: | 10.1111/exd.12458 |
| Popis: | Caspase-14, an important proteinase involved in filaggrin catabolism, is mainly active in terminally differentiating keratinocytes, where it is required for the generation of skin natural moisturizing factors (NMFs). Consequently, caspase-14 deficient epidermis is characterized by reduced levels of NMFs such as urocanic acid and 2-pyrrolidone-5-carboxylic acid. Patients suffering from filaggrin deficiency are prone to develop atopic dermatitis, which is accompanied with increased microbial burden. Among several reasons, this effect could be due to a decrease in filaggrin breakdown products. In this study, we found that caspase-14(-/-) mice show enhanced antibacterial response compared to wild-type mice when challenged with bacteria. Therefore, we compared the microbial communities between wild-type and caspase-14(-/-) mice by sequencing of bacterial 16S ribosomal RNA genes. We observed that caspase-14 ablation leads to an increase in bacterial richness and diversity during steadystate conditions. Although both wild-type and caspase-14(-/-) skin were dominated by the Firmicutes phylum, the Staphylococcaceae family was reduced in caspase-14(-/-) mice. Altogether, our data demonstrated that caspase-14 deficiency causes the imbalance of the skin-resident bacterial communities. |
| Databáze: | OpenAIRE |
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