Identification of bacterial strains isolated from the Mediterranean sea exhibiting different abilities of biofilm formation
| Autor: | Fabrice Armougom, Linda Guentas-Dombrowsky, Florence Brian-Jaisson, Yves Blache, Maëlle Molmeret, Annick Ortalo-Magné |
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| Přispěvatelé: | Laboratoire Matériaux Polymères Interfaces Environnement Marin - EA 4323 (MAPIEM), Université de Toulon (UTLN) |
| Rok vydání: | 2014 |
| Předmět: |
DNA
Bacterial Biodiversity Soil Science Zoology Bacterial Adhesion 03 medical and health sciences Marine bacteriophage Mediterranean sea Water column Microbial ecology RNA Ribosomal 16S Mediterranean Sea Seawater 14. Life underwater ComputingMilieux_MISCELLANEOUS Ecosystem Phylogeny Ecology Evolution Behavior and Systematics 030304 developmental biology 0303 health sciences Bacteria Ecology biology 030306 microbiology Biofilm Nucleic Acid Hybridization Sequence Analysis DNA biology.organism_classification Biofilms [SDE]Environmental Sciences France |
| Zdroj: | Microbial Ecology Microbial Ecology, 2014, 68 (1), pp.94--110. ⟨10.1007/s00248-013-0342-9⟩ |
| DOI: | 10.1007/s00248-013-0342-9⟩ |
| Popis: | The Mediterranean Sea has rarely been investigated for the characterization of marine bacteria as compared to other marine environments such as the Atlantic or Pacific Ocean. Bacteria recovered from inert surfaces are poorly studied in these environments, when it has been shown that the community structure of attached bacteria can be dissimilar from that of planktonic bacteria present in the water column. The objectives of this study were to identify and characterize marine bacteria isolated from biofilms developed on inert surfaces immersed in the Mediterranean Sea and to evaluate their capacity to form a biofilm in vitro. Here, 13 marine bacterial strains have been isolated from different supports immersed in seawater in the Bay of Toulon (France). Phylogenetic analysis and different biological and physico-chemical properties have been investigated. Among the 13 strains recovered, 8 different genera and 12 different species were identified including 2 isolates of a novel bacterial species that we named Persicivirga mediterranea and whose genus had never been isolated from the Mediterranean Sea. Shewanella sp. and Pseudoalteromonas sp. were the most preponderant genera recovered in our conditions. The phenotypical characterization revealed that one isolate belonging to the Polaribacter genus differed from all the other ones by its hydrophobic properties and poor ability to form biofilms in vitro. Identifying and characterizing species isolated from seawater including from Mediterranean ecosystems could be helpful for example, to understand some aspects of bacterial biodiversity and to further study the mechanisms of biofilm (and biofouling) development in conditions approaching those of the marine environment. |
| Databáze: | OpenAIRE |
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