Cardiovascular Response of Rat Aorta to Di-(2-ethylhexyl) Phthalate (DEHP) Exposure
| Autor: | Melissa Mariana, Joana Feiteiro, Elisa Cairrao |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine endocrine system medicine.medical_specialty Vascular smooth muscle Contraction (grammar) Calcium Channels L-Type Myocytes Smooth Muscle In Vitro Techniques 010501 environmental sciences Toxicology 01 natural sciences Muscle Smooth Vascular Cell Line Membrane Potentials 03 medical and health sciences chemistry.chemical_compound Plasticizers Diethylhexyl Phthalate Internal medicine medicine.artery medicine Animals Calcium Signaling Patch clamp Rats Wistar Molecular Biology Aorta 0105 earth and related environmental sciences Dose-Response Relationship Drug Voltage-dependent calcium channel Phthalate Calcium Channel Blockers Vasodilation 030104 developmental biology Endocrinology chemistry Cardiology and Cardiovascular Medicine Homeostasis Hormone |
| Zdroj: | Cardiovascular Toxicology. 18:356-364 |
| ISSN: | 1559-0259 1530-7905 |
| Popis: | Phthalates are one of the main constituents of plastic, reaching up to 40% of the total plastic weight, and their main function is to impart flexibility/elasticity to polymers that would otherwise be rigid. Phthalates are known as endocrine disruptors, since they can interfere with hormone homeostasis. Regarding the cardiovascular system, it was already shown the effects of di-(2-ethylhexyl) phthalate (DEHP) exposure with significant changes in several calcium-handling proteins and an increase in the blood pressure of mice offspring, suggesting that DEHP leads to vasocontraction. However, the mechanisms involved were not elucidated yet. The aim of this study is to analyse the involvement of calcium channels in the effects induced by DEHP on vascular smooth muscle cells. Endothelium-denuded aorta artery rings were prepared from male Wistar rats and incubated in an organ bath, and the whole-cell configuration of Patch Clamp technique was used to measure the activity of L-type Ca2+ channels (LTCC) in A7r5 cells. Overall, DEHP caused relaxation on KCl-induced contraction at higher concentrations and inhibited the basal and BAY K8644-stimulated calcium current, indicating that this drug blocks LTCC. These results suggest that DEHP induces relaxation on vascular smooth muscle cells due to the inhibition of calcium channels. |
| Databáze: | OpenAIRE |
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