Hair Follicular Expression and Function of Group X Secreted Phospholipase A2 in Mouse Skin
| Autor: | Yoshitaka Taketomi, Hiroki Nakanishi, Toshiharu Ishii, Yoshimi Miki, Yoshikazu Ishimoto, Yukio Ishikawa, Kazutaka Ikeda, Kiyoko Fukami, Yuki Isogai, Noriko Suzuki, Yasumasa Nishito, Kohji Hanasaki, Makoto Murakami, Kei Yamamoto, Tetsuyuki Kobayashi, Seiko Masuda, Ryo Taguchi, Yasunori Yokota, Kiyokazu Morioka, Hiroyasu Sato |
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| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
Biology Outer root sheath Biochemistry Gene Expression Regulation Enzymologic Melanin Mice Phospholipase A2 Hair cycle Internal medicine medicine Animals Homeostasis Phospholipases A2 Secretory Molecular Biology Melanins Mice Knockout Phospholipase A integumentary system Epidermis (botany) Alopecia Cell Biology Hair follicle Lipids Epithelium Enzyme Activation medicine.anatomical_structure Endocrinology Fatty Acids Unsaturated Phosphatidylcholines Prostaglandins biology.protein sense organs Hair Follicle |
| Zdroj: | Journal of Biological Chemistry. 286:11616-11631 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m110.206714 |
| Popis: | Although perturbed lipid metabolism can often lead to skin abnormality, the role of phospholipase A(2) (PLA(2)) in skin homeostasis is poorly understood. In the present study we found that group X-secreted PLA(2) (sPLA(2)-X) was expressed in the outermost epithelium of hair follicles in synchrony with the anagen phase of hair cycling. Transgenic mice overexpressing sPLA(2)-X (PLA2G10-Tg) displayed alopecia, which was accompanied by hair follicle distortion with reduced expression of genes related to hair development, during a postnatal hair cycle. Additionally, the epidermis and sebaceous glands of PLA2G10-Tg skin were hyperplasic. Proteolytic activation of sPLA(2)-X in PLA2G10-Tg skin was accompanied by preferential hydrolysis of phosphatidylethanolamine species with polyunsaturated fatty acids as well as elevated production of some if not all eicosanoids. Importantly, the skin of Pla2g10-deficient mice had abnormal hair follicles with noticeable reduction in a subset of hair genes, a hypoplasic outer root sheath, a reduced number of melanin granules, and unexpected up-regulation of prostanoid synthesis. Collectively, our study highlights the spatiotemporal expression of sPLA(2)-X in hair follicles, the presence of skin-specific machinery leading to sPLA(2)-X activation, a functional link of sPLA(2)-X with hair follicle homeostasis, and compartmentalization of the prostanoid pathway in hair follicles and epidermis. |
| Databáze: | OpenAIRE |
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