βPS-Integrin acts downstream of Innexin 2 in modulating stretched cell morphogenesis in the Drosophila ovary
| Autor: | Yu Chia Chen, Yi Chia Huang, Yu Yang Chen, Tsu Wei Wang, Liang Hsuan Tsao, Kuan Han Chen, Tsung Han Yeh, Ping Yen Wu, Jenn Yah Yu |
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| Rok vydání: | 2021 |
| Předmět: |
AcademicSubjects/SCI01140
Integrins follicle cells AcademicSubjects/SCI00010 integrin Morphogenesis morphogenesis Innexin QH426-470 Biology AcademicSubjects/SCI01180 Connexins 03 medical and health sciences 0302 clinical medicine Microtubule Genetics Animals Drosophila Proteins Molecular Biology Genetics (clinical) 030304 developmental biology Investigation 0303 health sciences Gene knockdown Cell morphogenesis oogenesis Ovary Gap junction Signal transducing adaptor protein stretched cells Cell biology AcademicSubjects/SCI00960 Drosophila Female Signal transduction 030217 neurology & neurosurgery microtubule |
| Zdroj: | G3: Genes|Genomes|Genetics G3: Genes, Genomes, Genetics, Vol 11, Iss 9 (2021) |
| ISSN: | 2160-1836 |
| Popis: | During oogenesis, a group of specialized follicle cells, known as stretched cells (StCs), flatten drastically from cuboidal to squamous shape. While morphogenesis of epithelia is critical for organogenesis, genes and signaling pathways involved in this process remain to be revealed. In addition to formation of gap junctions for intercellular exchange of small molecules, gap junction proteins form channels or act as adaptor proteins to regulate various cellular behaviors. In invertebrates, gap junction proteins are Innexins. Knockdown of Innexin 2 but not other Innexins expressed in follicle cells attenuates StC morphogenesis. Interestingly, blocking of gap junctions with an inhibitor carbenoxolone does not affect StC morphogenesis, suggesting that Innexin 2 might control StCs flattening in a gap-junction-independent manner. An excessive level of βPS-Integrin encoded by myospheroid is detected in Innexin 2 mutant cells specifically during StC morphogenesis. Simultaneous knockdown of Innexin 2 and myospheroid partially rescues the morphogenetic defect resulted from Innexin 2 knockdown. Furthermore, reduction of βPS-Integrin is sufficient to induce early StCs flattening. Taken together, our data suggest that βPS-Integrin acts downstream of Innexin 2 in modulating StCs morphogenesis. |
| Databáze: | OpenAIRE |
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