Characterization of P1 promoter activity of the beta-galactoside alpha2,6-sialyltransferase I gene (siat 1) in cervical and hepatic cancer cell lines
| Autor: | Verónica Vallejo-Ruiz, Gerardo Santos-López, Lorena Milflores-Flores, Julio Reyes-Leyva, Lourdes Millán-Pérez |
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| Rok vydání: | 2012 |
| Předmět: |
Gene isoform
Carcinoma Hepatocellular Sp1 Transcription Factor Molecular Sequence Data Gene Expression Uterine Cervical Neoplasms Biology General Biochemistry Genetics and Molecular Biology Antigens CD Cell Line Tumor Gene expression Transcriptional regulation RNA Isoforms Humans Luciferase RNA Messenger Promoter Regions Genetic Gene Binding Sites Base Sequence Liver Neoplasms Promoter General Medicine Hep G2 Cells Molecular biology Sialyltransferases Cell culture Cancer cell Hepatocyte Nuclear Factor 1 Mutation Female General Agricultural and Biological Sciences |
| Zdroj: | Journal of biosciences. 37(2) |
| ISSN: | 0973-7138 |
| Popis: | The level of beta-galactoside alpha2,6-sialyltransferase I (ST6Gal I) mRNA, encoded by the gene siat1, is increased in malignant tissues. Expression is regulated by different promoters - P1, P2 and P3 - generating three mRNA isoforms H, X and YZ. In cervical cancer tissue the mRNA isoform H, which results from P1 promoter activity, is increased. To study the regulation of P1 promoter, different constructs from P1 promoter were evaluated by luciferase assays in cervical and hepatic cell lines. Deletion of a fragment of 1048 bp (-89 to +24 bp) increased 5- and 3-fold the promoter activity in C33A and HepG2 cell lines, respectively. The minimal region with promoter activity was a 37 bp fragment in C33A cells. The activity of this region does not require the presence of an initiator sequence. In HepG2 cells the minimal promoter activity was detected in the 66 bp fragment. Sp1 (-32) mutation increased the promoter activity only in HepG2 cells. HNF1 mutation decreased promoter activity in HepG2 cell line but not in C33A cells. We identified a large region that plays a negative regulation role. The regulation of promoter activity is cell type specific. Our study provides new insights into the complex transcriptional regulation of siat1 gene. |
| Databáze: | OpenAIRE |
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