Albatross/FBF1 contributes to both centriole duplication and centrosome separation
| Autor: | Tohru Kiyono, Masaki Inagaki, Akihito Inoko, Naoki Goshima, Tatsuo Miyamoto, Shinya Matsuura, Yuko Hayashi, Tomoki Yano |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Centriole Cell Cycle Proteins Centrosome cycle Albatross Protein Serine-Threonine Kinases Biology Mice Phosphoserine 03 medical and health sciences Protein Domains Proto-Oncogene Proteins Ciliogenesis Serine Genetics Animals Humans Phosphorylation Mitosis Adaptor Proteins Signal Transducing Centrioles Centrosome Cell Biology Cell biology Mice Inbred C57BL HEK293 Cells 030104 developmental biology NIH 3T3 Cells Centrosome separation Multipolar spindles HeLa Cells Protein Binding |
| Zdroj: | Genes to Cells. 23:1023-1042 |
| ISSN: | 1365-2443 1356-9597 |
| DOI: | 10.1111/gtc.12648 |
| Popis: | The centrosome is a small but important organelle that participates in centriole duplication, spindle formation, and ciliogenesis. Each event is regulated by key enzymatic reactions, but how these processes are integrated remains unknown. Recent studies have reported that ciliogenesis is controlled by distal appendage proteins such as FBF1, also known as Albatross. However, the precise role of Albatross in the centrosome cycle, including centriole duplication and centrosome separation, remains to be determined. Here, we report a novel function for Albatross at the proximal ends of centrioles. Using Albatross monospecific antibodies, full-length constructs, and siRNAs for rescue experiments, we found that Albatross mediates centriole duplication by recruiting HsSAS-6, a cartwheel protein of centrioles. Moreover, Albatross participates in centrosome separation during mitosis by recruiting Plk1 to residue S348 of Albatross after its phosphorylation. Taken together, our results show that Albatross is a novel protein that spatiotemporally integrates different aspects of centrosome function, namely ciliogenesis, centriole duplication, and centrosome separation. |
| Databáze: | OpenAIRE |
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