Autometallographic tracing of mercury in frog liver
Autor: | G Danscher, N. S. Loumbourdis |
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Rok vydání: | 2003 |
Předmět: |
MERCURE
Health Toxicology and Mutagenesis chemistry.chemical_element Metal toxicity Biology Toxicology digestive system chemistry.chemical_compound Blood plasma medicine Animals Rana ridibunda Histocytochemistry General Medicine Anatomy Glutathione Mercury Pollution Molecular biology Mercury (element) Red blood cell medicine.anatomical_structure chemistry Liver Hepatocyte Environmental Pollutants Female Selenium Environmental Monitoring |
Zdroj: | Loumbourdis, N S & Danscher, G 2004, ' Autometallographic tracing of mercury in frog liver ' Environ. Pollut., vol. 129, no. 2, pp. 299-304 . |
ISSN: | 0269-7491 |
Popis: | The distribution of mercury in the liver of the frog Rana ridibunda with the autometallographic method was investigated. The mercury specific autometallographic (HgS/SeAMG) technique is a sensitive histochemical approach for tracing mercury in tissues from mercury-exposed organisms. Mercury accumulates in vivo as mercury sulphur/mercury selenium nanocrystals that can be silver-enhanced. Thus, only a fraction of the Hg can be visualized. Six animals were exposed for one day and another group of six animals for 6 days in 1 ppm mercury (as HgCI2 ) dissolved in fresh water. A third group of six animals, served as controls, were sacrificed the day of arrival at the laboratory. First, mercury appears in the blood plasma and erythrocytes. Next, mercury moves to hepatocytes and in the apical part of the cells, that facing bile canaliculi. In a next step, mercury appears in the endothelial and Kupffer cells. It seems likely that, the mercury of hepatocytes moves through bile canaliculi to the gut, most probably bound to glutathione and/or other similar ligands. Most probably, the endothelial and Kupffer cells comprise the first line of defense against metal toxicity. |
Databáze: | OpenAIRE |
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