The Role of Granulocytes in Colony Stimulation by Human White Blood Cells in Agar Cultures
| Autor: | H. Heimpel, Bernhard Kubanek, Peter Kern, Wolfgang Heit |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Ficoll
Hematology Biology Colony-stimulating factor Hemolysis Peripheral blood mononuclear cell Granulopoiesis Molecular biology In vitro Clone Cells Culture Media Hematopoiesis Agar Haematopoiesis Red blood cell medicine.anatomical_structure Colony-Stimulating Factors In vivo Immunology Leukocytes medicine Humans Cell Division Cells Cultured Granulocytes |
| Zdroj: | Scandinavian Journal of Haematology. 18:105-112 |
| ISSN: | 0036-553X |
| DOI: | 10.1111/j.1600-0609.1977.tb02079.x |
| Popis: | The effect of polymorphonuclear granulocytes (PMN) on colony stimulating activity (CSA) was studied in double layer cultures of human Ficoll Isopaque separated white blood cells (mononuclear cells = MNC). Previously published data have been confirmed that granulocytes are able to enhance or inhibit MNC derived CSA. Further analysis of the mode of action of PMN in vitro indicates that the enhancing activity ascribed to granulocytes coincides with low CSA in MNC basal layers. In contrast, in cultures with high levels of CSA as provided by lysed red blood cell enhancement rather than concentrations of PMN are sufficient to induce inhibition of colony growth. A very similar effect to that achieved with basal layer derived CSA could be obtained with conditioned media of PMN and MNC short term liquid cultures. The data indicate, that enhancement and inhibition of colony growth reflect a specific reactivity of granulocytes (PMN) to a given CSA level in the cultures. These findings are discussed in terms of a speculative role of PMN in a negative feed back control mechanism regulating granulopoiesis in vivo. |
| Databáze: | OpenAIRE |
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