High glucose induces activation of the local renin‑angiotensin system in glomerular endothelial cells
| Autor: | Tan Qi Lou, Hui Peng, Yan‑Fang Xing, Ming Li, Zeng Chun Ye, Peng‑Li Luo, Can Ming Li |
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| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
medicine.medical_specialty Angiotensinogen Biology Kidney Biochemistry Receptor Angiotensin Type 2 Receptor Angiotensin Type 1 Renin-Angiotensin System Western blot Internal medicine Renin–angiotensin system Renin Genetics medicine Extracellular Animals Humans Diabetic Nephropathies RNA Messenger Receptor Molecular Biology Cells Cultured medicine.diagnostic_test Angiotensin II Chymase Kidney metabolism Endothelial Cells Rats Endocrinology Glucose Oncology Molecular Medicine hormones hormone substitutes and hormone antagonists Intracellular |
| Zdroj: | Molecular medicine reports. 9(2) |
| ISSN: | 1791-3004 |
| Popis: | Activation of the intrarenal renin‑angiotensin system (RAS), which has been identified in podocytes and mesangial cells, is a novel mechanism in the progression of diabetic kidney disease (DKD). The present study aimed to identify the local RAS in glomerular endothelial cells (GEnCs). Rat GEnCs were stimulated by culture medium containing 30 mmol/l glucose for 12, 24, 48 and 72 h. Angiotensin II (Ang II) concentrations in cell lysates and culture media were examined by ELISA and mRNA levels of angiotensinogen and renin in cell lysates were analyzed by quantitative polymerase chain reaction. Ang II type 1 receptor (AT1R), Ang II type 2 receptor (AT2R), renin and angiotensinogen levels in cell lysates were determined by western blot analysis. Localization of intracellular AT1R, AT2R, angiotensinogen and renin was identified by confocal immunofluorescence microscopy. Consequently, high glucose (HG) increased intracellular and extracellular Ang II levels. Captopril and chymostatin (inhibitor of chymase, an enzyme that converts Ang I to Ang II) were able to antagonize HG‑induced Ang II generation. Moreover, HG increased angiotensinogen production in GEnCs and reduced renin mRNA expression without altering renin protein production. However, HG decreased AT1R levels and resulted in AT2R shifting from the nuclear to perinuclear region in GEnCs. In conclusion, HG activated the intracellular RAS in rat GEnCs and the underlying mechanism may involve angiotensin‑converting enzyme (ACE) and non‑ACE pathways. The effects of HG on GEnCs may also involve the substrate and receptors of Ang II. |
| Databáze: | OpenAIRE |
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