Patterns of large-scale genomic variation in virulent and avirulent Burkholderia species
| Autor: | Fiona Rodrigues, Kim Chong Ng, Catherine Ong, May Ann Lee, Hweeling Chong, Dongling Wang, Patrick Tan, Chia Huey Ooi |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Melioidosis
Burkholderia Nitrogen Iron Molecular Sequence Data Virulence Biology Genome Microbiology Open Reading Frames Biological Warfare Genetics medicine Letters ORFS Genetics (clinical) Oligonucleotide Array Sequence Analysis Burkholderia pseudomallei Polysaccharides Bacterial Glanders Genetic Variation Sequence Analysis DNA bacterial infections and mycoses biology.organism_classification medicine.disease Biological Evolution Bacterial Typing Techniques Quorum sensing Genes Bacterial bacteria Sequence Alignment Gene Deletion |
| Zdroj: | Genome Research. 14:2295-2307 |
| ISSN: | 1088-9051 |
| DOI: | 10.1101/gr.1608904 |
| Popis: | The human diseases melioidosis and glanders are caused by the bacteria Burkholderia pseudomallei and B. mallei respectively, and both species are regarded as potential biowarfare agents. We used B. pseudomallei DNA microarrays to compare the genomes of several clinical and environmental isolates of B. pseudomallei, B. mallei, and B. thailandensis, a closely related but avirulent species. Open reading frames (ORFs) deleted between the three species were associated with diverse cellular functions, including nitrogen and iron metabolism, quorum sensing, and polysaccharide production. Deleted ORFs in B. mallei exhibited significant genomic clustering, whereas deletions in B. thailandensis were more uniformly dispersed, suggesting that B. mallei and B. thailandensis may have diverged from B. pseudomallei and each other via distinct mechanisms. The genomes of independent B. pseudomallei isolates were highly conserved with a large-scale variance of less than 3% between isolates, and at least three distinct molecular subtypes could be defined. An analysis of subtype-specific genomic regions suggests that DNA loss has played an important role in the evolutionary radiation of B. pseudomallei in the natural environment. Our results raise several hypotheses concerning the possible mechanisms underlying the diverse biological properties exhibited by members of the Burkholderia family. |
| Databáze: | OpenAIRE |
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