Stanniocalcin-1 is a Modifier of Oxygen-Induced Retinopathy Severity
| Autor: | Colin Andrew Bretz, Michael P. Fautsch, David Sheikh-Hamad, Alan D. Marmorstein, Lauren A Dalvin, Mary Elizabeth Hartnett, Gavin W. Roddy, Cheryl R. Hann, Ricky Z Cui |
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| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Angiogenesis medicine.medical_treatment Inflammation medicine.disease_cause Neuroprotection Article Rats Sprague-Dawley Mice 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Animals Retinopathy of Prematurity Cells Cultured Glycoproteins Mice Knockout business.industry Growth factor Retinal Retinopathy of prematurity medicine.disease eye diseases Sensory Systems Rats Up-Regulation Oxygen Disease Models Animal Oxidative Stress Ophthalmology Endocrinology Animals Newborn Gene Expression Regulation chemistry 030221 ophthalmology & optometry sense organs medicine.symptom business 030217 neurology & neurosurgery Oxidative stress Signal Transduction Retinopathy |
| Zdroj: | Curr Eye Res |
| ISSN: | 1460-2202 0271-3683 |
| Popis: | PURPOSE/AIM: Abnormal activation of signaling pathways related to angiogenesis, inflammation, and oxidative stress has been implicated in the pathophysiology of retinopathy of prematurity (ROP), a leading cause of blindness in pre-term infants. Therapies for ROP include laser and anti-vascular endothelial growth factor agents. However, these therapies have side effects, and even with adequate treatment, visual acuity can be impaired. Novel therapeutic options are needed. Stanniocalcin-1 (STC-1) is neuroprotective protein with anti-inflammatory and anti-oxidative stress properties. Rodent models of oxygen-induced retinopathy (OIR) were selected to determine whether STC-1 plays a role in the development of OIR. MATERIALS AND METHODS: STC-1 gene and protein expression was first evaluated in the Sprague Dawley rat OIR model that is most similar to human ROP. OIR was then induced in wild-type and Stc-1(−/−) mice. Retinas were isolated and evaluated for avascular and neovascular area on retinal flat mounts. Quantification of gene expression by quantitative real-time PCR was performed. VEGF was assayed by ELISA in media obtained from induced pluripotent stem cell derived retinal pigment epithelial (iPS-RPE) cells following treatment with recombinant STC-1. RESULTS: STC-1 was significantly upregulated in a rat model of OIR compared to room air controls at the gene (P |
| Databáze: | OpenAIRE |
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