Metabolic proteomics of the liver and mammary gland during lactation
| Autor: | Christine Stockum, Pisana Rawson, Sarah D. Berry, Stephen R. Davis, H. E. Ward, Klaus Lehnert, Danyl McLauchlan, Bhagyashree Manivannan, Lifeng Peng, T. William Jordan, Russell G. Snell |
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| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Proteome Mammary gland Biophysics Biology Biochemistry chemistry.chemical_compound Mammary Glands Animal Internal medicine Lactation medicine Glycerol Animals Glycolysis Lactose chemistry.chemical_classification Lipid metabolism Endocrinology medicine.anatomical_structure Enzyme Gluconeogenesis chemistry Liver Organ Specificity Cattle Female |
| Zdroj: | Journal of proteomics. 75(14) |
| ISSN: | 1876-7737 |
| Popis: | The liver and the mammary gland have complementary metabolic roles during lactation. Glucose synthesized by the liver is released into the circulation and is taken up by the mammary gland where major metabolic products of glucose include milk sugar (lactose) and the glycerol backbone of milk fat (triglycerides). Hepatic synthesis of glucose is often accompanied by β-oxidation in that organ to provide energy for glucose synthesis, while mammary gland synthesizes rather than oxidizes fat during lactation. We have therefore compared enzyme abundances between the liver and mammary gland of lactating Friesian cows where metabolic output is well established. Quantitative differences in protein amount were assessed using two-dimensional differential in-gel electrophoresis. As predicted, the abundances of enzymes catalysing gluconeogenesis and β-oxidation were greatest in the liver, and enzyme abundances in mammary tissue were consistent with fat synthesis rather than β-oxidation. |
| Databáze: | OpenAIRE |
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