β-carotene inhibits UVA-induced matrix metalloprotease 1 and 10 expression in keratinocytes by a singlet oxygen-dependent mechanism
| Autor: | Christopher Lankin, Karin Wertz, Petra Buchwald Hunziker, Nicole Seifert, Adrian Wyss, Regina Goralczyk, Georges Riss |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Keratinocytes
Ultraviolet Rays medicine.drug_class Photoaging Retinoic acid Retinoid X receptor Biology Biochemistry Antioxidants Cell Line chemistry.chemical_compound Matrix Metalloproteinase 10 Downregulation and upregulation Physiology (medical) medicine Humans Retinoid DNA Primers Oligonucleotide Array Sequence Analysis Regulation of gene expression Analysis of Variance Base Sequence Singlet Oxygen Reverse Transcriptase Polymerase Chain Reaction Metalloendopeptidases Biological Transport beta Carotene medicine.disease Molecular biology HaCaT chemistry Cell culture Sunlight RNA Epidermis Matrix Metalloproteinase 1 |
| Zdroj: | Free Radical Biology and Medicine. 37:654-670 |
| ISSN: | 0891-5849 |
| Popis: | UVA exposure causes skin photoaging by singlet oxygen (1)O(2)-mediated induction of, e.g., matrix metalloproteases (MMPs). We assessed whether pretreatment with beta-carotene, a (1)O(2) quencher and retinoic acid (RA) precursor, interferes with UVA-induced gene regulation. HaCaT keratinocytes were precultured with beta-carotene at physiological concentrations (0.5, 1.5, and 3.0 microM) prior to exposure to UVA from a Hönle solar simulator (270 kJ/m(2)). HaCaT cells accumulated beta-carotene in a time- and dose-dependent manner. UVA irradiation massively reduced the cellular beta-carotene content. Beta-carotene suppressed UVA-induction of MMP-1, MMP-3, and MMP-10, three major matrix metalloproteases involved in photoaging. We show that regulation by not only MMP-1, but also MMP-10, involves (1)O(2)-dependent mechanisms. Beta-carotene dose-dependently quenched (1)O(2)-mediated induction of MMP-1 and MMP-10. Thus, as in chemical solvent systems, beta-carotene quenches (1)O(2) also in living cells. Vitamin E did not cooperate with beta-carotene to further inhibit MMP induction. HaCaT cells produced weak retinoid activity from beta-carotene, as demonstrated by mild upregulation of RAR beta and activation of an RARE-dependent reporter gene. Beta-carotene did not regulate the genes encoding other RARs, RXRs, or the two beta-carotene cleavage enzymes. These results demonstrate that beta-carotene acts photoprotectively, and that this effect is mediated by (1)O(2) quenching. |
| Databáze: | OpenAIRE |
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