Reflectance aptasensor based on metal salphen label for rapid and facile determination of insulin
| Autor: | Goh Choo Ta, Lee Yook Heng, Ling Ling Tan, Bahariah Khalid, Nurul Huda Abd Karim, Mahirah Taib |
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| Rok vydání: | 2020 |
| Předmět: |
Time Factors
Aptamer medicine.medical_treatment Enzyme-Linked Immunosorbent Assay Biosensing Techniques 02 engineering and technology Phenylenediamines 01 natural sciences Analytical Chemistry Metal chemistry.chemical_compound Limit of Detection Organometallic Compounds Side chain medicine Humans Insulin Schiff Bases Detection limit Chromatography Chemistry 010401 analytical chemistry Optical Devices Aptamers Nucleotide Hydrogen-Ion Concentration Silicon Dioxide 021001 nanoscience & nanotechnology 0104 chemical sciences Covalent bond visual_art visual_art.visual_art_medium Glutaraldehyde 0210 nano-technology Porosity Biosensor |
| Zdroj: | Talanta. 207:120321 |
| ISSN: | 0039-9140 |
| Popis: | An optical aptasensor-based sensing platform for rapid insulin detection was fabricated. Aminated porous silica microparticles (PSiMPs) were synthesized via a facile mini-emulsion method to provide large surface area for covalent immobilization of insulin-binding DNA aptamer (IGA3) by glutaraldehyde cross-linking protocol. A Nickel-salphen type complex with piperidine side chain [Ni(II)-SP] was synthesized with a simple one-pot reaction, and functionalized as an optical label due to strong π-π interaction between aromatic carbons of G-quadruplex DNA aptamer and planar aromatic groups of Ni(II)-SP to form the immobilized IGA3-Ni(II)-SP complex, i.e. the dye-labeled aptamer, thereby bringing yellow colouration to the immobilized G-quartet plane. Optical characterization of aptasensor towards insulin binding was carried out with a fiber optic reflectance spectrophotometer. The maximum reflectance intensity of the immobilized IGA3-Ni(II)-SP complex at 656 nm decreased upon binding with insulin as aptasensor changed to brownish orange colouration in the background. This allows optical detection of insulin as the colour change of aptasensor is dependent on the insulin concentration. The linear detection range of the aptasensor is obtained from 10 to 50 μIU mL−1 (R2 = 0.9757), which conformed to the normal fasting insulin levels in human with a limit of detection (LOD) at 3.71 μIU mL−1. The aptasensor showed fast response time of 40 min and long shelf life stability of >3 weeks. Insulin detection using healthy human serums with informed consent provided by participants suggests the DNA aptamer biosensor was in good agreement with ELISA standard method using BIOMATIK Human INS (Insulin) ELISA Kit. |
| Databáze: | OpenAIRE |
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