Cytocompatibility, Membrane Disruption, and siRNA Delivery using Environmentally Responsive Cationic Nanogels
| Autor: | David S. Spencer, Aaliyah B. Shodeinde, Hannah R. Hodges, David W. Beckman, Nicholas A. Peppas, Bryan C. Luu |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Chemistry Cationic polymerization Pharmaceutical Science Nanogels 02 engineering and technology Transfection 021001 nanoscience & nanotechnology In vitro Article 03 medical and health sciences Membrane Cations Self-healing hydrogels PEG ratio Biophysics Nucleic acid Nanoparticles RNA Small Interfering 0210 nano-technology Cytotoxicity 030304 developmental biology |
| Zdroj: | J Control Release |
| Popis: | Advances in the formulation of nucleic acid-based therapeutics have rendered them a promising avenue for treating diverse ailments. Nonetheless, clinical translation of these therapies is hindered by a lack of strategies to ensure the delivery of these nucleic acids in a safe, efficacious manner with the required spatial and temporal control. To this aim, environmentally responsive hydrogels are of interest due to their ability to provide the desired characteristics of a protective carrier for siRNA delivery. Previous work in our laboratory has demonstrated the ability to synthesize nanoparticle formulations with targeted pK(a), swelling, and surface PEG density. Here, a library of nanoparticle formulations was assessed on their in vitro toxicity, hemolytic capacity, siRNA loading, and gene-silencing efficacy. Successful candidates exhibited the lowest degrees of cytotoxicity, pH-dependent membrane disruption potential, the highest siRNA loading, and the highest transfection efficacies. |
| Databáze: | OpenAIRE |
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