Inhibition of growth and induction of differentiation of metastatic melanoma cells in vitro by genistein: chemosensitivity is regulated by cellular p53
| Autor: | J Kichina, A Green, Sikha Rauth |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Cancer Research
Cell division Tyrosinase Cellular differentiation Genistein Antineoplastic Agents Biology chemistry.chemical_compound Tumor Cells Cultured Humans Melanoma Cell growth Monophenol Monooxygenase food and beverages Cell Differentiation Protein-Tyrosine Kinases Isoflavones In vitro Oncology chemistry Biochemistry Cell culture Apoptosis Cancer research Tumor Suppressor Protein p53 Cell Division Research Article DNA Damage |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | We have investigated the effect of the soybean isoflavone genistein on the growth and differentiation of human melanoma cells. Four human melanoma cell lines, either completely lacking or containing different levels of wild-type p53, were treated with genistein in vitro in culture. It has been found that genistein significantly inhibited cell growth and that the chemosensitivity might depend on cellular p53 content. Specifically, the data suggest that high levels of wild-type p53 expression make cells resistant to genistein's growth-inhibitory action. Further support for this observation came from the stable transfection studies in which p53 transfectants expressing high levels of wild-type p53 became resistant to genistein. With respect to cell differentiation, our study showed that genistein increased melanin content and tyrosinase activity and caused the cells to form dendrite-like structures. Cells lacking p53 responded more than cells with p53 to dendrite-like structure formation. We also observed that genistein-induced differentiation involved an increase in tyrosinase mRNA level; the mechanisms by which genistein increases tyrosinase transcripts remain to be elucidated. Genistein treatment of the melanoma cell lines resulted in cell cycle arrest at G2/M check point and no significant apoptosis was observed. Images Figure 1 Figure 3 Figure 4 Figure 5 |
| Databáze: | OpenAIRE |
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