High-performance liquid chromatographic assay for identification and quantitation of nucleotides in lymphocytes and malignant lymphoblasts
| Autor: | E. D. A. M. Schretlen, John M. van Baal, Jan A.J.M. Bakkeren, Chris H.M.M. De Bruyn, Ronney A. De Abreu |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
chemistry.chemical_classification
Chromatography Leukemia Nucleotides Guanosine Cytidine General Chemistry Xanthosine Adenosine Uridine chemistry.chemical_compound chemistry Biochemistry Bone Marrow Reference Values medicine Humans Nucleotide NAD+ kinase Lymphocytes Inosine Child Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of chromatography. 227(1) |
| Popis: | A method for the identification and quantitation of nucleotide pools in lymphocytes and leukemic blasts is described. Separation of these metabolites was performed by anion-exchange high-performance liquid chromatography using a pH and concentration gradient consisting of several linear steps. The mono-, di- and triphosphates of adenosine, cytidine, guanosine, inosine, uridine and xanthosine could conveniently be separated together with NAD+, cyclic AMP, NADP+ and uridinediphosphoglucose (UDPG). In addition, data on the accuracy and precision of the method are given and its potentials for use in the analysis of nucleotide pools in leukemic lymphoblasts are illustrated. |
| Databáze: | OpenAIRE |
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