Molecular characterization of Mycobacterium tuberculosis isolated in the State of Parana in southern Brazil
| Autor: | Marcelo Malaghini, Afrânio Lineu Kritski, Sonia Regina Brockelt, Marion Burger, Vanete Thomaz-Soccol |
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| Rok vydání: | 2007 |
| Předmět: |
Microbiology (medical)
medicine.medical_specialty Tuberculosis Genotype Immunology Biology Microbiology Genetic analysis Polymerase Chain Reaction Mycobacterium tuberculosis Bacterial Proteins Polymorphism (computer science) Molecular genetics medicine Humans Tuberculosis Pulmonary Molecular Epidemiology UPGMA biology.organism_classification medicine.disease Virology DNA Fingerprinting Bacterial Typing Techniques Infectious Diseases Sputum medicine.symptom Brazil Polymorphism Restriction Fragment Length |
| Zdroj: | Tuberculosis (Edinburgh, Scotland). 89(1) |
| ISSN: | 1873-281X |
| Popis: | Summary Sequence IS 6110 has been successfully used throughout the world for characterizing the Mycobacterium tuberculosis lineages. The aim of this study was to obtain data about circulating strains of M. tuberculosis in patients from the State of Parana in southern Brazil. Sixty-two clinical specimens obtained from sputum, bronchial aspirate, biopsy and urine from 62 patients clinically diagnosed with tuberculosis and admitted to the SUS-Brazil – The Brazilian Centralized Health Service System – were genotyped by the mixed-linker PCR DNA fingerprinting technique. The analysis demonstrated that the number of copies of the IS 6110 sequence per isolates varied from four to 13 bands, with an average number of 8.5. From this, 93% of the isolates presented multiple copies. Isolates with no copies of the IS 6110 element were not observed. The genetic analysis by UPGMA grouped the 62 isolates by similarity into three different groups: the first group contained two strains, the second was composed of 23, and the third, a more heterogeneous group, contained 37 isolates. Only two isolates (3.2%) formed a cluster; in other words, they presented a pattern of polymorphism with similarity above 95%. Such findings suggest that in the State of Parana, illness predominantly develops through reactivation of the latent infection as opposed to exogenous transmission. The methodology used (mixed-linker PCR DNA fingerprinting) allowed for 93.5% differentiation of the isolates tested, and proved to be a powerful tool for differentiation in the molecular genotyping of M. tuberculosis . |
| Databáze: | OpenAIRE |
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