Rapid milk group classification by 1H NMR analysis of Le and H epitopes in human milk oligosaccharide donor samples
| Autor: | Lubbert Dijkhuizen, Ellen J M van Leusen-van Kan, Sander S. van Leeuwen, Johannis P. Kamerling, Gerrit J. Gerwig, Ruud J. W. Schoemaker |
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| Přispěvatelé: | Host-Microbe Interactions |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
chemistry.chemical_classification
Glycan Magnetic Resonance Spectroscopy Milk Human biology Oligosaccharides Oligosaccharide Biochemistry Epitope Fucose Epitopes chemistry.chemical_compound Lewis Blood Group Antigens fluids and secretions chemistry Group (periodic table) biology.protein Proton NMR Humans Protons Glycoprotein Fucosylation |
| Zdroj: | Glycobiology, 24(8), 728-739. Oxford University Press |
| ISSN: | 0959-6658 |
| Popis: | Human milk oligosaccharides (HMOs) are a major constituent of human breast milk and play an important role in reducing the risk of infections in infants. The structures of these HMOs show similarities with blood group antigens in protein glycosylation, in particular in relation to fucosylation in Lewis blood group-type epitopes, matching the maternal pattern. Previously, based on the Secretor and Lewis blood group system, four milk groups have been defined, i.e. Lewis-positive Secretors, Lewis-positive non-Secretors, Lewis-negative Secretors and Lewis-negative non-Secretors. Here, a rapid one-dimensional (1)H nuclear magnetic resonance (NMR) analysis method is presented that identifies the presence/absence of (α1-2)-, (α1-3)- and (α1-4)-linked fucose residues in HMO samples, affording the essential information to attribute different HMO samples to a specific milk group. The developed method is based on the NMR structural-reporter-group concept earlier established for glycoprotein glycans. Further evaluation of the data obtained from the analysis of 36 HMO samples shows that within each of the four milk groups the relative levels of the different fucosylation epitopes can greatly vary. The data also allow a separation of the Lewis-positive Secretor milk group into two sub-groups. |
| Databáze: | OpenAIRE |
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