The cytoprotective effect of α-tocopherol and daidzein against d-galactosamine–induced oxidative damage in the rat liver
| Autor: | Philip R. Dash, Roy Sherwood, Heidi Koivisto, Bernard Portmann, Helen Wiseman, Raj Srirajaskanthan, Seppo Parkkila, Onni Niemelä, M C Y Wong, Victor R. Preedy, James W. Wilson, Keith D. Trick, Mary R. L'Abbé |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Vitamin medicine.medical_specialty Necrosis Endocrinology Diabetes and Metabolism alpha-Tocopherol Galactosamine medicine.disease_cause Superoxide dismutase chemistry.chemical_compound Endocrinology Malondialdehyde Internal medicine medicine Animals Rats Wistar Glutathione Peroxidase biology Superoxide Dismutase Daidzein Albumin medicine.disease Isoflavones Rats Oxidative Stress Liver chemistry Cytoprotection biology.protein Steatosis medicine.symptom Oxidative stress |
| Zdroj: | Metabolism. 56:865-875 |
| ISSN: | 0026-0495 |
| Popis: | We hypothesized that the hepatotoxicity that develops after the induction of oxidative stress (induced by d-galactosamine [GalN]) can be ameliorated by alpha-tocopherol (ATC) and the soy isoflavone daidzein. To test this, we ranked and assigned male Wistar rats into 6 groups, which involved pretreatment (ATC or daidzein) for 1 hour followed by treatment (GalN) for 23 hours. Histopathologic analysis showed that GalN administration induced marked necrosis (P < .001), steatosis (P < .001), both lobular and portal inflammations (P < .001), overall histopathologic score (P < .001), and activation of caspase-3 in the liver (P < .001). Immunohistochemical staining of malondialdehyde-protein adducts, a measure of oxidative stress, was increased in response to GalN (P < .001). Paradoxically, there were increases in total (P < .05) and cytosolic superoxide dismutase (P < .001) activities after GalN administration, indicative of an up-regulation of antioxidant defenses. The concentration of total protein (P < .001), albumin (P < .01), and globulin fractions (P < .001) in the plasma, as well as the activity of aspartate aminotransferase (P < .001), was significantly perturbed after GalN treatment, reflective of overall acute hepatic injury. Administration of daidzein showed a significant amelioration of the Ga1N-induced increase in malondialdehyde-protein adducts (P < .01) and cytosolic superoxide dismutase activities (P < .01) in the liver. However, all other variables were not significantly altered in response to daidzein. In response to ATC pretreatment, the total histopathologic score (P < .05), degree of necrosis (P < .05), and both lobular (P < .05) and portal (P = .05) inflammations were significantly ameliorated. To conclude, both daidzein and ATC protect the liver against oxidative damage possibly via different pathways. |
| Databáze: | OpenAIRE |
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