The use of ex-vivo liver perfusion circuit in sheep model: Preliminary report
| Autor: | Ahmed Al-Jammali, Falah H. Al-Mohanna, Saleh I. Al Abbad, Yazeed M. Alsebayel, Yasser Elsheikh, M. Al-Sebayel, Yaser H. Al Nemry, Hamad M. Al-Bahli, Norah K. Al-Zeer |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Machine perfusion Sheep business.industry Swine Portal venous pressure Saudi Arabia Hemodynamics General Medicine Liver Transplantation Perfusion medicine.anatomical_structure Blood pressure Volume (thermodynamics) Liver Internal medicine medicine Cardiology Animals business Ex vivo Artery |
| Zdroj: | Saudi medical journal. 42(12) |
| ISSN: | 0379-5284 |
| Popis: | Objectives: To describe a novel animal model for ex-vivo liver perfusion. Methods: This study was carried out at King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia, between September 2016 and January 2019. We assembled a perfusion circuit operated by a continuous pressure-driven arterial pump with continuous portal and arterial pressure and volume measurements. We used normothermic oxygenated perfusate. The livers used were retrieved from the sheep. Results: Ex-vivo continuous perfusion of the liver was achieved for up to 9 hours with stable pressure and volume in both hepatic artery and portal vein. In 4 experiments the arterial pressure was kept in a range of 48-52 mmHg with a mean of 51.75±4.31 resulting in arterial volume at steady state of 223.5±48.25 ml/minute (95% confidence level). At steady state the mean portal pressure was 16.25±1.45 mmHg with a mean volume of 854±313.75 ml/minute (95% confidence level). Bile production was observed during the perfusion period. Hemodynamic parameters were similar to the physiological parameters observed in normothermic perfusion model of the porcine liver. Conclusion: A normothermic oxygenated ex-vivo perfusion circuit was successfully constructed using the sheep liver. A sustainable functional circuit with physiological hemodynamic parameters was achieved. Further study on sheep model seems to be feasible. |
| Databáze: | OpenAIRE |
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