Label-free and reagentless electrochemical detection of PCR fragments using self-assembled quinone derivative monolayer: application to Mycobacterium tuberculosis
| Autor: | Peter E. Nielsen, Edgar Abadia, Minh-Chau Pham, Q.D. Zhang, Benoît Piro, Gregory March, L.V. Hai, L.D. Tran, Christophe Sola, S. Reisberg, Vincent Noël |
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| Přispěvatelé: | Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
MESH: Mycobacterium tuberculosis
Base Pair Mismatch Oligonucleotides 02 engineering and technology Biosensing Techniques MESH: Base Sequence 01 natural sciences Polymerase Chain Reaction law.invention MESH: Nucleic Acid Hybridization chemistry.chemical_compound law MESH: Oligonucleotides Electrochemistry Polymerase chain reaction Quinones Nucleic Acid Hybridization General Medicine 021001 nanoscience & nanotechnology MESH: Electrochemical Techniques 3. Good health [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology 0210 nano-technology MESH: Biosensing Techniques Biotechnology DNA Bacterial Biomedical Engineering Biophysics Biology 010402 general chemistry Sensitivity and Specificity MESH: Quinones Mycobacterium tuberculosis Nucleic acid thermodynamics [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Sulfhydryl Compounds MESH: Sulfhydryl Compounds MESH: Base Pair Mismatch Base Sequence Oligonucleotide MESH: Polymerase Chain Reaction Electrochemical Techniques biology.organism_classification Molecular biology Combinatorial chemistry MESH: DNA Bacterial MESH: Sensitivity and Specificity 0104 chemical sciences chemistry Hydroxynaphthoquinone Biosensor DNA |
| Zdroj: | Biosensors and Bioelectronics Biosensors and Bioelectronics, Elsevier, 2012, 32 (1), pp.163-8. ⟨10.1016/j.bios.2011.11.048⟩ |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2011.11.048⟩ |
| Popis: | International audience; We report a signal-on, label-free and reagentless electrochemical DNA biosensor, based on a mixed self-assembled monolayer of thiolated hydroxynaphthoquinone and thiolated oligonucleotide. Electrochemical changes resulting from hybridization were evidenced with oligonucleotide targets (as models), as well as with polymerase chain reaction (PCR) products related to different lineages of Mycobacterium tuberculosis strains. With pure oligonucleotides, this system achieves high sensitivity (∼300 pM of DNA target, i.e. 30 fmol in a 100 μL sample) and excellent selectivity, allowing to detect a single mismatch on a sequence of 20 bases. With PCR products, current changes are specific to the bacterial strain from which the PCR fragment is produced. In addition, the sensor response is of the signal-on type, giving a positive signal change upon hybridization, and therefore does not suffer from false positive responses due to non-specific adsorption of DNA. |
| Databáze: | OpenAIRE |
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