Reciprocal expression oflin-41and the microRNAslet-7andmir-125during mouse embryogenesis
| Autor: | Aurora Esquela-Kerscher, Frank J. Slack, Betsy R. Schulman |
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| Rok vydání: | 2005 |
| Předmět: |
Molecular Sequence Data
Cell fate determination Biology Article Conserved sequence Mice microRNA Animals Limb development Amino Acid Sequence Cloning Molecular Base Pairing Gene Transcription factor Conserved Sequence In Situ Hybridization DNA Primers Regulation of gene expression Genetics Binding Sites Base Sequence fungi Gene Expression Regulation Developmental Extremities Sequence Analysis DNA Blotting Northern Embryo Mammalian MicroRNAs Gene Components Heterochrony Transcription Factors Developmental Biology |
| Zdroj: | Developmental Dynamics. 234:1046-1054 |
| ISSN: | 1097-0177 1058-8388 |
| Popis: | In C. elegans, heterochronic genes control the timing of cell fate determination during development. Two heterochronic genes, let-7 and lin-4, encode microRNAs (miRNAs) that down-regulate a third heterochronic gene lin-41 by binding to complementary sites in its 3'UTR. let-7 and lin-4 are conserved in mammals. Here we report the cloning and sequencing of mammalian lin-41 orthologs. We find that mouse and human lin-41 genes contain predicted conserved complementary sites for let-7 and the lin-4 ortholog, mir-125, in their 3'UTRs. Mouse lin-41 (Mlin-41) is temporally expressed in developing mouse embryos, most dramatically in the limb buds. Mlin-41 is down-regulated during mid-embryogenesis at the time when mouse let-7c and mir-125 RNA levels are up-regulated. Our results suggest that mammalian lin-41 is temporally regulated by miRNAs in order to direct key developmental events such as limb formation. |
| Databáze: | OpenAIRE |
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