Neuroprotective effect of Alpinia galanga (L.) fractions on Aβ(25-35) induced amnesia in mice
| Autor: | Y. Narsimha Reddy, J. C. Hanish Singh, S. Sathesh Kumar, Prakash V. Diwan, J.C. Nisha, Veerachamy Alagarsamy |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Antioxidant food.ingredient medicine.medical_treatment Alpinia galanga Pharmacology medicine.disease_cause Neuroprotection Antioxidants chemistry.chemical_compound Mice food Cognition Alzheimer Disease Memory Drug Discovery medicine Animals Enzyme Inhibitors Maze Learning Nootropic Agents Amyloid beta-Peptides biology Traditional medicine Behavior Animal business.industry Plant Extracts Cell Membrane Neurotoxicity Brain Alpinia biology.organism_classification medicine.disease Acetylcholinesterase Disease Models Animal Oxidative Stress Neuroprotective Agents chemistry Zingiberaceae Amnesia Sodium-Potassium-Exchanging ATPase business Oxidative stress Rhizome Phytotherapy |
| Zdroj: | Journal of ethnopharmacology. 138(1) |
| ISSN: | 1872-7573 |
| Popis: | Ethnopharmacological relevance The rhizomes of Alpinia galanga (L.) Willd (Zingiberaceae), a ginger substitute for flavouring food was traditionally used as nervine tonic and stimulant. Aim of the study This investigation is designed to screen cognitive improvement of Alpinia galanga (AG) fractions in Alzheimer's type of amnesia in mice induced by Aβ(25–35). Materials and methods Alzheimer's disease induced mice treated with fractions (n-hexane, chloroform and ethyl acetate) of AG in 200 and 400 mg/kg. Neurotoxicity was induced by intracerebroventricular injection of Aβ(25–35) on the 14th day of 21 days drug treatment. Open field and water maze were carried to determine habituation memory and hippocampal memory. Na+/K+-ATPase, acetylcholinesterase (AChE) and antioxidant enzymes (SOD, GPx, catalase and vitamin C) were determined in brain tissue homogenate to estimate the brain biochemical changes and its anti-amnesic potential with intensity of oxidative stress signaling. Further bioactive (chloroform) fraction was eluted through column chromatography to identify the lead molecules. Results Increased habituation memory and decreased escape latency in behavioral parameter are the indicative of the cognitive enhancement after treatment with Alpinia galanga fractions. Increment in Na+/K+-ATPase and antioxidant activity depicts brain membrane integrity improvement and free radical scavenging property. AChE level was decreased to improve the cognition by enhancing cholinergic transmission. Conclusion Anti-amnesic effect was exerted by various fractions of Alpinia galanga. Among all fractions, preeminent neuroprotection was exerted by chloroform fraction, which has compound, 1′δ-1′-acetoxyeugenol acetate and it may be a potential therapeutic agent for Alzheimer's type of amnesia. These results further motivate us to explore the activity of lead compound's anti-amnesic effect on transgenic mice model of AD. |
| Databáze: | OpenAIRE |
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