Full‐thickness tissue engineered oral mucosa for genitourinary reconstruction: A comparison of different collagen‐based biodegradable membranes
| Autor: | Ronald E. Unger, Bilal Al-Nawas, Annette Hasenburg, R Schwab, Celine Pfeifer, Walburgis Brenner, Sandra Nezi-Cahn, Martin Heller, Stefan Walenta |
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| Rok vydání: | 2020 |
| Předmět: |
Collagen Type IV
Scaffold Materials science Swine Biomedical Engineering Tenascin Biocompatible Materials Matrix (biology) Fibroblast migration Biomaterials 03 medical and health sciences 0302 clinical medicine Tissue engineering Absorbable Implants Materials Testing medicine Animals Viability assay Oral mucosa Fibroblast Cells Cultured Tissue Engineering Tissue Scaffolds biology Keratin-13 Mouth Mucosa Epithelial Cells Membranes Artificial 030206 dentistry Fibroblasts Plastic Surgery Procedures Coculture Techniques Urogenital Surgical Procedures Cell biology medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein |
| Zdroj: | Journal of Biomedical Materials Research Part B: Applied Biomaterials. 109:572-583 |
| ISSN: | 1552-4981 1552-4973 |
| DOI: | 10.1002/jbm.b.34724 |
| Popis: | Tissue engineering is a method of growing importance regarding clinical application in the genitourinary region. One of the key factors in successfully development of an artificially tissue engineered mucosa equivalent (TEOM) is the optimal choice of the scaffold. Collagen scaffolds are regarded as gold standard in dermal tissue reconstruction. Four distinct collagen scaffolds were evaluated for the ability to support the development of an organotypical tissue architecture. TEOMs were established by seeding cocultures of primary oral epithelial cells and fibroblasts on four distinct collagen membranes. Cell viability was assessed by MTT-assay. The 3D architecture and functionality of the tissue engineered oral mucosa equivalents were evaluated by confocal laser-scanning microscopy and immunostaining. Cell viability was reduced on the TissuFoil E® membrane. A multi-stratified epithelial layer was established on all four materials, however the TEOMs on the Bio-Gide® scaffold showed the best fibroblast differentiation, secretion of tenascin and fibroblast migration into the membrane. The TEOMs generated on Bio-Gide® scaffold exhibited the optimal cellular organization into a cellular 3D network. Thus, the Bio-Gide® scaffold is a suitable matrix for engineering of mucosa substitutes in vitro. |
| Databáze: | OpenAIRE |
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