Thiolated poly(2-hydroxyethyl methacrylate) hydrogels as a degradable biocompatible scaffold for tissue engineering
| Autor: | Šárka Kubinová, Vladimír Proks, Miroslav Vetrik, Bohumila Podhorská, Ognen Pop-Georgievski, Hana Macková, Zhansaya Kaberova, Olga Janoušková, Jan Kučka, Helena Hlídková, Daniel Horák, Vitalii Patsula |
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| Rok vydání: | 2021 |
| Předmět: |
Materials science
Bioengineering Biocompatible Materials macromolecular substances Methacrylate complex mixtures Biomaterials Tissue engineering Animals Polyhydroxyethyl Methacrylate chemistry.chemical_classification Tissue Engineering technology industry and agriculture Chain transfer Hydrogels Mesenchymal Stem Cells Polymer Raft Rats Chemical engineering chemistry Polymerization Mechanics of Materials Drug delivery Self-healing hydrogels Methacrylates |
| Zdroj: | Materials scienceengineering. C, Materials for biological applications. 131 |
| ISSN: | 1873-0191 |
| Popis: | Research of degradable hydrogel polymeric materials exhibiting high water content and mechanical properties resembling tissues is crucial not only in drug delivery systems but also in tissue engineering, medical devices, and biomedical-healthcare sensors. Therefore, we newly offer development of hydrogels based on poly(2-hydroxyethyl methacrylate-co-2-(acetylthio) ethyl methacrylate-co-2-methacryloyloxyethyl phosphorylcholine) [P(HEMA-ATEMA-MPC)] and optimization of their mechanical and in vitro and in vivo degradability. P(HEMA-ATEMA-MPC) hydrogels differed in chemical composition, degree of crosslinking, and starting molar mass of polymers (15, 19, and 30 kDa). Polymer precursors were synthesized by a reversible addition fragmentation chain transfer (RAFT) polymerization using 2-(acetylthio)ethyl methacrylate containing protected thiol groups, which enabled crosslinking and gel formation. Elastic modulus of hydrogels increased with the degree of crosslinking (Slaughter et al., 2009) [1] . In vitro and in vivo controlled degradation was confirmed using glutathione and subcutaneous implantation of hydrogels in rats, respectively. We proved that the hydrogels with higher degree of crosslinking retarded the degradation. Also, albumin, γ-globulin, and fibrinogen adsorption on P(HEMA-ATEMA-MPC) hydrogel surface was tested, to simulate adsorption in living organism. Rat mesenchymal stromal cell adhesion on hydrogels was improved by the presence of RGDS peptide and laminin on the hydrogels. We found that rat mesenchymal stromal cells proliferated better on laminin-coated hydrogels than on RGDS-modified ones. |
| Databáze: | OpenAIRE |
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