In Vivo Imaging of Single Tumor Cells in Fast-Flowing Bloodstream Using Near-Infrared Quantum Dots and Time-Gated Imaging
| Autor: | Nicolas Lequeux, Sophie Pezet, Alexandra Fragola, Sophie Bouccara, Vincent Loriette, Thomas Pons |
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| Přispěvatelé: | Laboratoire de Physique et d'Etude des Matériaux (UMR 8213) (LPEM), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Neurobiologie, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2019 |
| Předmět: |
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Erythrocytes Time Factors Photon Infrared Rays Surface Properties Single tumor General Physics and Astronomy 02 engineering and technology 010402 general chemistry 01 natural sciences Fluorescence Rats Sprague-Dawley Circulating tumor cell Cell Line Tumor Quantum Dots Animals Humans General Materials Science [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics ComputingMilieux_MISCELLANEOUS Chemistry Optical Imaging Near-infrared spectroscopy General Engineering 021001 nanoscience & nanotechnology Healthy Volunteers Rats 3. Good health 0104 chemical sciences Autofluorescence Spectrometry Fluorescence Quantum dot Biophysics Single-Cell Analysis 0210 nano-technology Preclinical imaging |
| Zdroj: | ACS Nano ACS Nano, American Chemical Society, 2019, 13 (3), pp.3125-3131. ⟨10.1021/acsnano.8b08463⟩ |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/acsnano.8b08463 |
| Popis: | Whereas in vivo fluorescence imaging of cells immobilized within tissues provides a valuable tool to a broad range of biological studies, it still lacks the sensitivity required to visualize isolated cells circulating fast in the bloodstream due, in particular, to the autofluorescence from endogenous fluorophores. Time-gated imaging of near-infrared emitting ZnCuInSe/ZnS quantum dots (QDs) with fluorescence lifetimes in the range of 150-300 ns enables the efficient rejection of fast autofluorescence photons and the selection of QD fluorescence photons, thus significantly increasing sensitivity. We labeled model erythrocytes as well as lymphoma cells using these QDs coated with a stable zwitterionic polymer surface chemistry. After reinjection in the bloodstream, we were able to image and count individual QD-labeled cells circulating at mm·s-1 velocities in blood vessels. |
| Databáze: | OpenAIRE |
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