ST3Gal III modulates breast cancer cell adhesion and invasion by altering the expression of invasion-related molecules
| Autor: | Chun-hui Xia, Hong‑Xia Cui, Yu-chun Wang, Yetong Shen, Hong-lan Wang, Juan Song, Hua Tian |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oncology Cancer Research medicine.medical_specialty beta-Galactoside alpha-2 3-Sialyltransferase Cell Lewis X Antigen Breast Neoplasms Biology Metastasis 03 medical and health sciences 0302 clinical medicine Breast cancer Cell Line Tumor Internal medicine Cell Adhesion Human Umbilical Vein Endothelial Cells medicine Humans Neoplasm Invasiveness Cell adhesion Oncogene Integrin beta1 Cancer Cell migration General Medicine Cell cycle medicine.disease Sialyltransferases Gene Expression Regulation Neoplastic 030104 developmental biology medicine.anatomical_structure Matrix Metalloproteinase 9 Cyclooxygenase 2 030220 oncology & carcinogenesis Cancer research Matrix Metalloproteinase 2 Female E-Selectin |
| Zdroj: | Oncology Reports. 36:3317-3324 |
| ISSN: | 1791-2431 1021-335X |
| DOI: | 10.3892/or.2016.5180 |
| Popis: | Changes in the carbohydrate structure on the surface of tumor cells is an important feature of cancer metastasis. The specific role of sialic acids in the glycoconjugate terminal has not yet been clearly elucidated in these processes. Previously, we reported that α2,3-sialic acid residues in breast cancer are associated with metastatic potential. The α2,3-sialyltransferase ST3Gal III, which adds α2,3-sialic acids to glycoproteins, is overexpressed in various tumors, and enzyme activity is correlated with tumor metastasis, yet its mechanistic role has not been fully evaluated. In the present study, we aimed to investigate the influence of ST3Gal III on key steps in the process of breast cancer metastasis. ST3Gal III-overexpressing and ST3Gal III-silenced breast cancer MDA-MB-231 cell lines were generated. They showed an increase or decrease in the tumor-associated antigen sialyl-Lewis X (SLeX). The E-selectin binding capacity of the transfectants was proportional to cell surface SLeX levels. Cell migration and invasion were positively correlated with ST3Gal III levels. Moreover, ST3Gal III expression modulated the protein expression of invasion-related molecules, including β1 integrin, matrix metalloproteinase (MMP)-2, MMP-9 and cyclooxygenase-2, which may account for the mechanism involved in the effects of ST3Gal III on breast cancer invasiveness. In conclusion, our findings in these novel models of ST3Gal III expression revealed a critical requirement for ST3Gal III in several steps of breast cancer metastasis. ST3Gal III modulates breast cancer cell adhesion and invasion by altering the expression of invasion-related molecules. This study provides novel insights into the mechanisms underlying metastasis and suggests a new target for the effective drug treatment of breast cancer metastasis. |
| Databáze: | OpenAIRE |
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