Construction of a rhamnose mutation in Bacillus anthracis affects adherence to macrophages but not virulence in guinea pigs
| Autor: | Joel A. Bozue, Lawrence R. Phillips, Itai Mendelson, Arthur M. Friedlander, Avigdor Shafferman, Christopher K. Cote, P.F. Fellows, Narayanan Parthasarathy |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Cytochalasin D
Rhamnose Guinea Pigs Mutant Virulence Microbiology Bacterial Adhesion Anthrax chemistry.chemical_compound Ames strain Animals Sequence Deletion Spores Bacterial biology Strain (chemistry) Macrophages fungi Exosporium biology.organism_classification Spore Bacillus anthracis Disease Models Animal Infectious Diseases chemistry Multigene Family Mutation Female |
| Zdroj: | Microbial Pathogenesis. 38:1-12 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2004.10.001 |
| Popis: | Carbohydrate analyses of whole-spore extracts have confirmed the presence of rhamnose in the spore of the fully virulent Ames strain of Bacillus anthracis. A gene cluster containing loci with high homology to the rhamnose biosynthetic genes, rmlACBD, was identified within the B. anthracis chromosome. The first gene of this cluster, rmlA, was inactivated by forming a merodiploid cointegrate using an internal fragment of the gene within the Ames strain of B. anthracis to construct the mutant strain Ames-JAB1. Carbohydrate analysis of spores from this mutant demonstrated the loss of rhamnose. When assaying for spore infection of macrophages, we detected a significant decrease in the recovery with the Ames-JAB1 strain compared to the recovery with the Ames wild-type strain. When pre-treating macrophages with cytochalasin-D, spores of the mutant were further hindered in recovery, indicating that the spores were not able to bind as well to the macrophages. However, in guinea pigs challenge experiments, no difference in virulence was observed between the mutant and wild-type strains. These results suggest that the incorporation of rhamnose into the spore coat of B. anthracis is required for optimal interaction with macrophages but is not required for full virulence in this animal model. |
| Databáze: | OpenAIRE |
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