In vitro studies of theophylline-induced changes in Na, K and Cl transport in hen (Gallus domesticus) colon suggesting bidirectional, basolateral NaK2Cl cotransport
| Autor: | B. G. Munck, Vibeke Andersen, Lars K Munck, Erik Skadhauge |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
Colon Chloride Ouabain Chlorides Theophylline Internal medicine medicine Animals Intestinal Mucosa Incubation Ion transporter Bumetanide Chemistry Sodium Biological Transport General Medicine Membrane transport Endocrinology Biophysics Potassium Female Cotransporter Chickens medicine.drug |
| Zdroj: | Comparative biochemistry and physiology. A, Comparative physiology. 96(1) |
| ISSN: | 0300-9629 |
| Popis: | 1. 1. In isolated mucosa from a NaCl-loaded hen theophylline stimulates both unidirectional chloride fluxes (JClmsand JClms). Conductive and electroneutral exchange processes, besides a bumetanidesensitive, rheogenic process contribute. 2. 2. The bumetanide-sensitive fraction of the theophylline-induced ΔJClms is sodium-dependent. 3. 3. Incubation in nominally K+-free solutions reduces the bumetanide-sensitive fraction ΔJClms more than treatment with ouabain. 4. 4. With respect to chloride the bumetanide-sensitive fraction of ΔJClms has a Hill coefficient of 1.93 ± 0.03, a Jmax of 12.9 ± 0.2 μ mol/cm 2 · hr and a K 1 2 of 73 ± 1 mmol/l . 5. 5. After ouabain treatment ΔJmsCl and ΔJmsCl are equally sensitive to bumetanide, while ΔJmsCl is bumetanide insensitive without ouabain treatment. |
| Databáze: | OpenAIRE |
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