Evidence that Extreme Dilutions of Paclitaxel and Docetaxel Alter Gene Expression of In Vitro Breast Cancer Cells
| Autor: | Celal Güven, Handan Akçakaya, Fahri Akbas, Sinem Şeker, Nurten Bahtiyar, İlhan Onaran |
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| Přispěvatelé: | AKBAŞ, FAHRİ |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Gene Expression Antineoplastic Agents Apoptosis Breast Neoplasms Pharmacology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor Gene expression medicine Humans Cytotoxicity Cell Proliferation Taxane Homeopathy Reverse transcription polymerase chain reaction 030104 developmental biology Complementary and alternative medicine Paclitaxel chemistry Docetaxel Cell culture 030220 oncology & carcinogenesis MCF-7 Cells Female Trypan blue the journal of the Faculty of Homeopathy cilt.107 ss.32-39 2018 [Şeker S. Güven C. Akçakaya H. Bahtiyar N. Akbaş F. Onaran İ. -Evidence that Extreme Dilutions of Paclitaxel and Docetaxel Alter Gene Expression of In Vitro Breast Cancer Cells.- Homeopathy] medicine.drug |
| Popis: | Background Gene expression analysis of cells treated with extreme dilutions or micro amounts of drugs has been used to provide useful suggestions about biological responses. However, most of the previous studies were performed on medicines being prepared from a variety of herbal and metal sources. This study investigated the effects of ultramolecular dilution of the taxane anti-cancer drugs, which are not commonly used in homeopathic medicines, on mRNA expression profiles of five key genes (p53, p21, COX-2, TUBB2A and TUBB3) in the breast cancer cell line MCF-7. Method MCF-7 cells were exposed to paclitaxel (Taxol) or docetaxel (Taxotere) preparations (6X, 5C and 15C dilutions prepared from pharmacological concentration of 25 nmol/L) for 72 hours. The cell culture groups were evaluated with the trypan blue dye exclusion method for the proliferation/cytotoxicity rates, immuno-staining β-tubulin for microtubule organization, and reverse transcription polymerase chain reaction for gene expression levels.Fold-change in gene expression was determined by the ΔΔCt method. Results The administration of diluted preparations had little or no cytotoxic effect on MCF-7 cells, but altered the expression of genes analyzed with a complex effect. According to the ΔΔCt method with a five-fold expression difference (p Conclusion Despite some limitations, our findings demonstrate that gene expression alterations also occur with ultra-high dilutions of taxane drugs. |
| Databáze: | OpenAIRE |
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