Laccase isoform diversity in basidiomycete Lentinus strigosus 1566: Potential for phenylpropanoid polymerization
| Autor: | A.S. Gaidina, N. B. Gasanov, Anna D. Shebanova, M. P. Kolomytseva, L. A. Golovleva, Zh. V. Renfeld, Joerg Pietruszka, J. Aschenbrenner, A.M. Chernykh, J.F. Rosengarten, Boris P. Baskunov, I.P. Pinchuk, N. M. Myasoedova, Thomas Classen |
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| Rok vydání: | 2019 |
| Předmět: |
Glycosylation
Propanols Lentinus strigosus 02 engineering and technology Biochemistry Polymerization 03 medical and health sciences Structural Biology Enzyme Stability Amino Acid Sequence Molecular Biology Incubation 030304 developmental biology Laccase 0303 health sciences Strain (chemistry) biology Phenylpropanoid Chemistry Basidiomycota Temperature General Medicine Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology biology.organism_classification Isoenzymes Transformation (genetics) Kinetics Metals 0210 nano-technology Selectivity |
| Zdroj: | International journal of biological macromolecules. 137 |
| ISSN: | 1879-0003 |
| Popis: | Three laccase isoforms with different physicochemical properties could be purified from culture liquid of basidiomycete Lentinus strigosus 1566 obtained during submerged cultivation. The purified laccases possessed individual selectivity in relation to different phenolic compounds. Laccases I, II, and III (59, 65, and 61 kDa respectively) were more active in acidic conditions at around 70 °C. However, in contrast to laccases I and II, laccase III retained its activity (8–30%) and stability during at least one week of incubation at neutral conditions that allows its biotechnological application carried out at neutral environment. The activation phenomena for some of the purified laccases from L. strigosus 1566 during incubation at high temperature, different pH, and sulfates is shown and discussed. According to MALDI-TOF analysis, laccases I and II are most closely related to the laccase of Panus rudis (AAR13230). Transformation of phenylpropanoids by the predominant laccases of L. strigosus 1566 to different polymers was demonstrated, indicating a great potential for producing novel pharmaceutical valuable analogues of lignans, stilbenes, flavonoids, and etc.. The studied laccases, which are products of the same strain, can become a convenient model for further studies of the structural mechanisms of the shift of T-/pH-optima, activation, and T-/pH-stability. |
| Databáze: | OpenAIRE |
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