Pre-induction of Hsp70 expression to protect the grooved carpet shell clam, Ruditapes decussatus, against Micrococcus luteus: A trained immunity strategy
| Autor: | Bahaa Abdella, Eman El-Wazzan, Doaa A. Ghareeb |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
animal structures
010501 environmental sciences Aquatic Science Plant disease resistance Oceanography 01 natural sciences lcsh:Aquaculture. Fisheries. Angling Microbiology Ruditapes decussatus Immune system Aquaculture Downregulation and upregulation Immunity Ecology Evolution Behavior and Systematics lcsh:Environmental sciences 0105 earth and related environmental sciences Water Science and Technology lcsh:SH1-691 lcsh:GE1-350 biology business.industry 04 agricultural and veterinary sciences biology.organism_classification Hsp70 040102 fisheries 0401 agriculture forestry and fisheries bacteria business Micrococcus luteus |
| Zdroj: | Egyptian Journal of Aquatic Research, Vol 46, Iss 1, Pp 79-84 (2020) |
| ISSN: | 1687-4285 |
| Popis: | Bacterial pathogens cause disease-associated mortality in Ruditapes decussatus, a model bivalve for aquaculture in Egypt. In this study, it was hypothesized that trained immunity could be achieved to enhance disease resistance against Micrococcus luteus by pre-inducing Hsp70 expression. Clams were challenged via a seawater bath with M. luteus at various concentrations (2.5 × 104, 5.0 × 104, and 7.5 × 104 CFU/ml), gill Hsp70 was quantified using ELISA and infected clams were compared to controls in two experiments (with one 17-day and two 17-day infection periods separated by a 7-day depuration period for recovery, respectively). Infection with M. luteus significantly altered Hsp70 expression compared to the control levels. Downregulation occurred at low concentrations and upregulation at the two higher concentrations. When used in the trained immunity trial, Hsp70 upregulation occurred in infected clams during the first infection and persisted during the 7-day recovery. Re-infection led to the increase of Hsp70 levels, which reached maximum values, and enhanced survival. The results confirmed that Hsp70 is involved in the immune response and disease resistance by protecting R. decussatus against re-infection with M. luteus. These results suggested the use of trained immunity as disease control strategy. Keywords: Bivalve aquaculture, Ruditapes decussatus, Hsp70, Disease control, Micrococcus luteus, Trained immunity |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|