Use of force spectroscopy to investigate the adhesion of living adherent cells
| Autor: | Harry Heinzelmann, Martha Liley, Raphaël Pugin, Nicolas Blondiaux, Gilles Weder, M. J. K. Klein, Nadège Matthey, Marta Giazzon |
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| Přispěvatelé: | University of Zurich, Weder, G |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Materials science
3104 Condensed Matter Physics Surface Properties Analytical chemistry 1607 Spectroscopy 610 Medicine & health 1603 Electrochemistry Microscopy Atomic Force 3T3 cells 170 Ethics Mice Microscopy Cell Adhesion Electrochemistry medicine Animals General Materials Science 10237 Institute of Biomedical Engineering Cell adhesion Spectroscopy Quartz Cell Proliferation Cell growth Substrate (chemistry) 3T3 Cells Surfaces and Interfaces Adhesion 3110 Surfaces and Interfaces Condensed Matter Physics 2500 General Materials Science Fibronectins medicine.anatomical_structure Biophysics |
| DOI: | 10.5167/uzh-43548 |
| Popis: | The use of force spectroscopy to study the adhesion of living fibroblasts to their culture substrate was investigated. Both primary fibroblasts (PEMF) and a continuous cell line (3T3) were studied on quartz surfaces. Using a fibronectin- coated AFM cantilever, it was possible to detach a large proportion of the 3T3 cells from the quartz surfaces. Their adhesion to the quartz surface and the effects of topography on this adhesion could be quantified. Three parameters characteristic of the adhesion were measured: the maximum force of detachment, the work of adhesion, and the distance of detachment. Few PEMF cells were detached under the same experimental conditions. The potential and limitations of this method in measuring cell/surface interactions for adherent cells are discussed. |
| Databáze: | OpenAIRE |
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