A novel DNA adjuvant, N3, enhances mucosal and systemic immune responses induced by HIV-1 DNA and peptide immunizations
| Autor: | Jorma Hinkula, Claudia Devito, Reinhold Benthin, Ulf Schröder, Bartek Zuber, Denise Ferreira, Britta Wahren |
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| Rok vydání: | 2006 |
| Předmět: |
medicine.medical_treatment
HIV Antibodies Biology Virus HIV Envelope Protein gp160 Feces Mice Plasmid Immune system Neutralization Tests Vaccines DNA medicine Animals Neutralizing antibody Immunity Mucosal Immunity Cellular General Veterinary General Immunology and Microbiology Public Health Environmental and Occupational Health biology.organism_classification Virology HIV Envelope Protein gp41 Immunoglobulin A Mice Inbred C57BL Infectious Diseases DNA Viral Vagina Lentivirus Immunology HIV-1 biology.protein Molecular Medicine Female Nasal administration Antibody Peptides Adjuvant |
| Zdroj: | Vaccine. 24:4494-4497 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2005.08.015 |
| Popis: | Aims The study was designed to evaluate a novel cationic lipid DNA adjuvant (N3) and its function for HIV-1gp160/rev DNA plasmid delivered intranasally. The primary N3/HIV-DNA plasmid immunizations were boosted intranasally with a gp41 peptide in a anionic L3 adjuvant. This novel prime-boost strategy of mucosal immunization provided a broad HIV-1 envelope specific immunity, and recognition of viruses of subtypes A, B and C. Conclusions Intranasal N3-adjuvanted gp160/rev DNA prime followed by one L3-peptide boosting immunization, induced broadly neutralizing antibodies against HIV-1 in the mucosa and systemically. The needle-free intranasal prime-boost strategy using two different adjuvant formulations reduced significantly the dose of DNA needed. |
| Databáze: | OpenAIRE |
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