Effects of inducible overexpression of DNp73α on cancer cell growth and response to treatment in vitro and in vivo
| Autor: | Eugenio Scanziani, Sergio Marchini, Massimo Broggini, E. Riccardi, Mirko Marabese, Federica Polato, Faina Vikhanskaya, Maria Antonietta Sabatino, Eleonora Marrazzo |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
DNA damage
Clone (cell biology) Gene Expression Biology Mice In vivo Neoplasms medicine Animals Humans Genes Tumor Suppressor neoplasms Molecular Biology Cell Proliferation Doxycycline Antibiotics Antineoplastic Tumor Suppressor Proteins Nuclear Proteins Tumor Protein p73 Cell Biology Transfection HCT116 Cells Molecular biology digestive system diseases In vitro DNA-Binding Proteins Phenotype Doxorubicin Cell culture Cancer cell biological phenomena cell phenomena and immunity DNA Damage medicine.drug |
| Zdroj: | Cell Death & Differentiation. 12:805-814 |
| ISSN: | 1476-5403 1350-9047 |
| DOI: | 10.1038/sj.cdd.4401622 |
| Popis: | The p73 gene has a complex regulation, which leads to the expression of different isoforms, often with opposite biological effects. We have generated in the human colocarcinoma cell line HCT116, expressing a wild-type p53, an inducible DNp73alpha expressing system. Two clones (HCT116/DN3 and HCT116/DN14), upon doxycycline addition, show a strong expression of DNp73alpha. In vitro the two DNp73alpha overexpressing clones grow at similar rate of the control transfected clone (HCT116/8a) and similarly respond to DNA damage. When injected in mice, HCT116/DN3, HCT116/DN14, and HCT116/8a cells grew similarly in the absence or presence of tetracycline. In HCT116/DN3 and HCT116/DN14 tumors, tetracycline induced a strong expression of DNp73alpha both as mRNA and protein. These results indicate that in this system the overexpression of the DNp73alpha does not induce a more aggressive phenotype and does not seem to be associated with a reduced response of the cells to treatment with anticancer agents. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|