Adipose-derived stem cell transplantation for therapeutic lymphangiogenesis in a mouse secondary lymphedema model
| Autor: | Shuhei Yoshida, Hiroshi Yoshimoto, Rodrigo Hamuy, Yuuichi Hamada, Sadanori Akita, Akiyoshi Hirano |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Embryology Pathology medicine.medical_specialty Secondary lymphedema Infrared Rays Green Fluorescent Proteins Vascular Endothelial Growth Factor C Biomedical Engineering Video Recording Adipose tissue Hindlimb Mice Cell transplantation Medicine Animals Regeneration Lymphedema Lymphangiogenesis In Situ Hybridization Fluorescence Glycoproteins Lymphatic Vessels business.industry Membrane Transport Proteins Vascular Endothelial Growth Factor Receptor-3 Immunohistochemistry Mice Inbred C57BL Disease Models Animal Lymphatic system Adipose Tissue Female medicine.symptom Stem cell business Stem Cell Transplantation |
| Zdroj: | Regenerative medicine. 10(5) |
| ISSN: | 1746-076X |
| Popis: | Aim: Secondary lymphedema is observed in common after postmalignancy treatment of the breast and the gynecologic organs but effective therapies are not established. Adipose-derived stem cells (ADSCs), which are pluripotent, regenerative in local injection, are tested for murine hindlimb secondary lymphedema by regenerative method. Methods & results: Mice were divided into four groups: no ADSCs, 1 × 106 ADSCs, 1 × 105 ADSCs and 1 × 104 ADSCs (each group, n = 20) in a stringent surgical resection and irradiation. Circumferential measurement, lymphatic flow assessment and quantification of lymphatic vessels were performed. Results: The numbers of lymphatic vessels by LYVE-1 immunohistochemistry, and VEGF-C- or VEGFR3-expressing cells were significantly increased in transplanted groups (p < 0.05). Conclusion: ADSCs can restore the lymphatic vascular network in secondary lymphedema with increased collecting vessels. |
| Databáze: | OpenAIRE |
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