A role for Gic1 and Gic2 in Cdc42 polarization
| Autor: | Christine N Daniels, Daniel J. Lew, Trevin R. Zyla |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0303 health sciences
biology Chemistry Saccharomyces cerevisiae macromolecular substances CDC42 GTPase biology.organism_classification 3. Good health Cell biology 03 medical and health sciences 0302 clinical medicine Cell polarity Cell cortex Guanine nucleotide exchange factor biological phenomena cell phenomena and immunity Cytoskeleton 030217 neurology & neurosurgery 030304 developmental biology Septin cytoskeleton |
| DOI: | 10.1101/407569 |
| Popis: | The conserved Rho-family GTPase Cdc42 is a master regulator of polarity establishment in many cell types. Cdc42 becomes activated and concentrated in a region of the cell cortex, and recruits a variety of effector proteins to that site. In turn, many effectors participate in regulation of cytoskeletal elements in order to remodel the cytoskeleton in a polarized manner. The budding yeast Saccharomyces cerevisiae has served as a tractable model system for studies of cell polarity. In yeast cells, Cdc42 polarization involves a positive feedback loop in which effectors called p21-activated kinases (PAKs) act to recruit a Cdc42-directed guanine nucleotide exchange factor (GEF), generating more GTP-Cdc42 in areas that already have GTP-Cdc42. The GTPase-interacting components (GICs) Gic1 and Gic2 are also Cdc42 effectors, and have been implicated in regulation of the actin and septin cytoskeleton. However, we report that cells lacking GICs are primarily defective in polarizing Cdc42 itself, suggesting that they act upstream as well as downstream of Cdc42 in yeast. Our findings suggest that feedback pathways involving GTPase effectors may be more prevalent than had been appreciated. |
| Databáze: | OpenAIRE |
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